CRISPR-U™ Gene Knockout Cell Line
Strategy
DNAJC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DNAJC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DNAJC2 |
| Gene id | 27000 |
| Organism | Homo sapiens |
| Official full symbol | DnaJ heat shock protein family (Hsp40) member C2 |
| Gene type | protein-coding |
| Also known as | MPHOSPH11, MPP11, ZRF1, ZUO1 |
| Summary | This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DNAJC2-203 | ENST00000379263.8 | 2291 | 621aa | Protein coding | CCDS43628 | Q99543-1 | NM_014377.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DNAJC2-201 | ENST00000249270.11 | 1846 | 568aa | Protein coding | CCDS47679 | Q99543-2 | - | TSL:1, GENCODE basic, |
| DNAJC2-204 | ENST00000412522.1 | 3033 | 118aa | Protein coding | - | F2Z3H0 | - | TSL:1, GENCODE basic, |
| DNAJC2-206 | ENST00000454277.5 | 854 | 188aa | Protein coding | - | C9IZ83 | - | CDS 3' incomplete, TSL:5, |
| DNAJC2-205 | ENST00000426036.6 | 729 | 243aa | Protein coding | - | H7C3L7 | - | CDS 5' and 3' incomplete, TSL:5, |
| DNAJC2-202 | ENST00000379257.7 | 942 | 167aa | Nonsense mediated decay | - | F2Z3N9 | - | TSL:1, |
| DNAJC2-211 | ENST00000492497.1 | 2167 | No protein | Retained intron | - | - | - | TSL:2, |
| DNAJC2-208 | ENST00000475065.5 | 1952 | No protein | Retained intron | - | - | - | TSL:1, |
| DNAJC2-207 | ENST00000464253.5 | 1809 | No protein | Retained intron | - | - | - | TSL:1, |
| DNAJC2-209 | ENST00000475090.1 | 480 | No protein | Retained intron | - | - | - | TSL:5, |
| DNAJC2-210 | ENST00000483637.5 | 373 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DNAJC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DNAJC2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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