CRISPR-U™ Gene Knockout Cell Line Strategy

DNAJC2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DNAJC2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DNAJC2
Gene id 27000
Organism Homo sapiens
Official full symbol DnaJ heat shock protein family (Hsp40) member C2
Gene type protein-coding
Also known as MPHOSPH11, MPP11, ZRF1, ZUO1
Summary This gene is a member of the M-phase phosphoprotein (MPP) family. The gene encodes a phosphoprotein with a J domain and a Myb DNA-binding domain which localizes to both the nucleus and the cytosol. The protein is capable of forming a heterodimeric complex that associates with ribosomes, acting as a molecular chaperone for nascent polypeptide chains as they exit the ribosome. This protein was identified as a leukemia-associated antigen and expression of the gene is upregulated in leukemic blasts. Also, chromosomal aberrations involving this gene are associated with primary head and neck squamous cell tumors. This gene has a pseudogene on chromosome 6. Alternatively spliced variants which encode different protein isoforms have been described.
Genomic regions Chromosome 7
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DNAJC2-203 ENST00000379263.8 2291 621aa Protein coding CCDS43628 Q99543-1 NM_014377.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DNAJC2-201 ENST00000249270.11 1846 568aa Protein coding CCDS47679 Q99543-2 - TSL:1, GENCODE basic,
DNAJC2-204 ENST00000412522.1 3033 118aa Protein coding - F2Z3H0 - TSL:1, GENCODE basic,
DNAJC2-206 ENST00000454277.5 854 188aa Protein coding - C9IZ83 - CDS 3' incomplete, TSL:5,
DNAJC2-205 ENST00000426036.6 729 243aa Protein coding - H7C3L7 - CDS 5' and 3' incomplete, TSL:5,
DNAJC2-202 ENST00000379257.7 942 167aa Nonsense mediated decay - F2Z3N9 - TSL:1,
DNAJC2-211 ENST00000492497.1 2167 No protein Retained intron - - - TSL:2,
DNAJC2-208 ENST00000475065.5 1952 No protein Retained intron - - - TSL:1,
DNAJC2-207 ENST00000464253.5 1809 No protein Retained intron - - - TSL:1,
DNAJC2-209 ENST00000475090.1 480 No protein Retained intron - - - TSL:5,
DNAJC2-210 ENST00000483637.5 373 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DNAJC2
This KO Strategy loading
Red Cotton™ Notes Gene DNAJC2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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