CRISPR-U™ Gene Knockout Cell Line Strategy

AMH Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AMH Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AMH
Gene id 268
Organism Homo sapiens
Official full symbol anti-Mullerian hormone
Gene type protein-coding
Also known as MIF, MIS
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active noncovalent complex. This complex binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in the male embryo that would otherwise differentiate into the uterus and fallopian tubes. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome.
Genomic regions Chromosome 19
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AMH-201 ENST00000221496.5 1809 560aa Protein coding CCDS12085 P03971 NM_000479.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
AMH-203 ENST00000592877.1 534 No protein Processed transcript - - - TSL:3,
AMH-202 ENST00000589313.2 1686 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AMH
This KO Strategy loading
Red Cotton™ Notes Gene AMH had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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