CRISPR-U™ Gene Knockout Cell Line
Strategy
AMH Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AMH Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AMH |
| Gene id | 268 |
| Organism | Homo sapiens |
| Official full symbol | anti-Mullerian hormone |
| Gene type | protein-coding |
| Also known as | MIF, MIS |
| Summary | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active noncovalent complex. This complex binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in the male embryo that would otherwise differentiate into the uterus and fallopian tubes. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AMH-201 | ENST00000221496.5 | 1809 | 560aa | Protein coding | CCDS12085 | P03971 | NM_000479.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| AMH-203 | ENST00000592877.1 | 534 | No protein | Processed transcript | - | - | - | TSL:3, |
| AMH-202 | ENST00000589313.2 | 1686 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AMH |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AMH had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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