CRISPR-U™ Gene Knockout Cell Line Strategy

GGCX Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GGCX Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GGCX
Gene id 2677
Organism Homo sapiens
Official full symbol gamma-glutamyl carboxylase
Gene type protein-coding
Also known as VKCFD1
Summary This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 2
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GGCX-201 ENST00000233838.9 7556 758aa Protein coding CCDS1978 P38435-1 NM_000821.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GGCX-205 ENST00000430215.7 2314 701aa Protein coding CCDS46353 P38435-2 - TSL:2, GENCODE basic,
GGCX-203 ENST00000423570.5 1339 123aa Nonsense mediated decay - F8WDU9 - TSL:1,
GGCX-202 ENST00000421496.5 540 66aa Nonsense mediated decay - F8WCF0 - TSL:4,
GGCX-204 ENST00000428479.3 533 73aa Nonsense mediated decay - F8WB98 - TSL:4,
GGCX-207 ENST00000473665.1 869 No protein Processed transcript - - - TSL:2,
GGCX-209 ENST00000482662.1 720 No protein Processed transcript - - - TSL:3,
GGCX-210 ENST00000496962.1 684 No protein Processed transcript - - - TSL:2,
GGCX-208 ENST00000481541.1 600 No protein Processed transcript - - - TSL:2,
GGCX-206 ENST00000465637.5 563 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GGCX
This KO Strategy loading
Red Cotton™ Notes Gene GGCX had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×