CRISPR-U™ Gene Knockout Cell Line
Strategy
GGCX Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GGCX Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GGCX |
| Gene id | 2677 |
| Organism | Homo sapiens |
| Official full symbol | gamma-glutamyl carboxylase |
| Gene type | protein-coding |
| Also known as | VKCFD1 |
| Summary | This gene encodes an integral membrane protein of the rough endoplasmic reticulum that carboxylates glutamate residues of vitamin K-dependent proteins to gamma carboxyl glutamate, a modification that is required for their activity. The vitamin K-dependent protein substrates have a propeptide that binds the enzyme, with carbon dioxide, dioxide, and reduced vitamin K acting as co-substrates. Vitamin K-dependent proteins affect a number of physiologic processes including blood coagulation, prevention of vascular calcification, and inflammation. Allelic variants of this gene have been associated with pseudoxanthoma elasticum-like disorder with associated multiple coagulation factor deficiency. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GGCX-201 | ENST00000233838.9 | 7556 | 758aa | Protein coding | CCDS1978 | P38435-1 | NM_000821.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GGCX-205 | ENST00000430215.7 | 2314 | 701aa | Protein coding | CCDS46353 | P38435-2 | - | TSL:2, GENCODE basic, |
| GGCX-203 | ENST00000423570.5 | 1339 | 123aa | Nonsense mediated decay | - | F8WDU9 | - | TSL:1, |
| GGCX-202 | ENST00000421496.5 | 540 | 66aa | Nonsense mediated decay | - | F8WCF0 | - | TSL:4, |
| GGCX-204 | ENST00000428479.3 | 533 | 73aa | Nonsense mediated decay | - | F8WB98 | - | TSL:4, |
| GGCX-207 | ENST00000473665.1 | 869 | No protein | Processed transcript | - | - | - | TSL:2, |
| GGCX-209 | ENST00000482662.1 | 720 | No protein | Processed transcript | - | - | - | TSL:3, |
| GGCX-210 | ENST00000496962.1 | 684 | No protein | Processed transcript | - | - | - | TSL:2, |
| GGCX-208 | ENST00000481541.1 | 600 | No protein | Processed transcript | - | - | - | TSL:2, |
| GGCX-206 | ENST00000465637.5 | 563 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GGCX |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GGCX had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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