CRISPR-U™ Gene Knockout Cell Line Strategy

GDI2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GDI2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GDI2
Gene id 2665
Organism Homo sapiens
Official full symbol GDP dissociation inhibitor 2
Gene type protein-coding
Also known as HEL-S-46e, RABGDIB
Summary GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI2 is ubiquitously expressed. The GDI2 gene contains many repetitive elements indicating that it may be prone to inversion/deletion rearrangements. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 10
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GDI2-203 ENST00000380191.9 2297 445aa Protein coding CCDS7071 P50395-1 NM_001494.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GDI2-202 ENST00000380181.7 1408 400aa Protein coding CCDS44352 P50395-2 - TSL:1, GENCODE basic,
GDI2-206 ENST00000456041.5 918 263aa Protein coding - Q5SX87 - CDS 3' incomplete, TSL:5,
GDI2-205 ENST00000447751.5 834 203aa Protein coding - Q5SX91 - CDS 5' incomplete, TSL:3,
GDI2-204 ENST00000418688.5 636 155aa Protein coding - Q5SX86 - CDS 3' incomplete, TSL:5,
GDI2-208 ENST00000608581.5 634 130aa Protein coding - V9GYF8 - CDS 3' incomplete, TSL:3,
GDI2-209 ENST00000609712.1 622 125aa Protein coding - V9GYJ7 - CDS 3' incomplete, TSL:3,
GDI2-201 ENST00000380127.5 584 148aa Protein coding - Q5SX90 - CDS 3' incomplete, TSL:3,
GDI2-207 ENST00000479928.1 2499 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GDI2
This KO Strategy loading
Red Cotton™ Notes Gene GDI2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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