CRISPR-U™ Gene Knockout Cell Line Strategy

PCOLCE2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PCOLCE2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PCOLCE2
Gene id 26577
Organism Homo sapiens
Official full symbol procollagen C-endopeptidase enhancer 2
Gene type protein-coding
Also known as PCPE2
Genomic regions Chromosome 3
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PCOLCE2-201 ENST00000295992.8 1896 415aa Protein coding CCDS3127 Q9UKZ9 NM_013363.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PCOLCE2-211 ENST00000648195.1 2022 401aa Protein coding - A0A3B3ITE8 - GENCODE basic,
PCOLCE2-208 ENST00000485766.1 1088 303aa Protein coding - C9JYX9 - TSL:5, GENCODE basic,
PCOLCE2-205 ENST00000480473.5 773 101aa Nonsense mediated decay - H7C520 - CDS 5' incomplete, TSL:5,
PCOLCE2-203 ENST00000470310.5 728 140aa Nonsense mediated decay - H7C5D5 - CDS 5' incomplete, TSL:5,
PCOLCE2-202 ENST00000461818.5 909 No protein Processed transcript - - - TSL:3,
PCOLCE2-210 ENST00000495732.5 704 No protein Processed transcript - - - TSL:3,
PCOLCE2-209 ENST00000493733.1 566 No protein Processed transcript - - - TSL:4,
PCOLCE2-204 ENST00000470795.5 967 No protein Retained intron - - - TSL:2,
PCOLCE2-206 ENST00000483454.1 666 No protein Retained intron - - - TSL:2,
PCOLCE2-207 ENST00000483986.1 538 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PCOLCE2
This KO Strategy loading
Red Cotton™ Notes Gene PCOLCE2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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