CRISPR-U™ Gene Knockout Cell Line
Strategy
PCOLCE2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PCOLCE2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PCOLCE2 |
| Gene id | 26577 |
| Organism | Homo sapiens |
| Official full symbol | procollagen C-endopeptidase enhancer 2 |
| Gene type | protein-coding |
| Also known as | PCPE2 |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PCOLCE2-201 | ENST00000295992.8 | 1896 | 415aa | Protein coding | CCDS3127 | Q9UKZ9 | NM_013363.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PCOLCE2-211 | ENST00000648195.1 | 2022 | 401aa | Protein coding | - | A0A3B3ITE8 | - | GENCODE basic, |
| PCOLCE2-208 | ENST00000485766.1 | 1088 | 303aa | Protein coding | - | C9JYX9 | - | TSL:5, GENCODE basic, |
| PCOLCE2-205 | ENST00000480473.5 | 773 | 101aa | Nonsense mediated decay | - | H7C520 | - | CDS 5' incomplete, TSL:5, |
| PCOLCE2-203 | ENST00000470310.5 | 728 | 140aa | Nonsense mediated decay | - | H7C5D5 | - | CDS 5' incomplete, TSL:5, |
| PCOLCE2-202 | ENST00000461818.5 | 909 | No protein | Processed transcript | - | - | - | TSL:3, |
| PCOLCE2-210 | ENST00000495732.5 | 704 | No protein | Processed transcript | - | - | - | TSL:3, |
| PCOLCE2-209 | ENST00000493733.1 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| PCOLCE2-204 | ENST00000470795.5 | 967 | No protein | Retained intron | - | - | - | TSL:2, |
| PCOLCE2-206 | ENST00000483454.1 | 666 | No protein | Retained intron | - | - | - | TSL:2, |
| PCOLCE2-207 | ENST00000483986.1 | 538 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PCOLCE2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PCOLCE2 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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