CRISPR-U™ Gene Knockout Cell Line
Strategy
AGO1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human AGO1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | AGO1 |
| Gene id | 26523 |
| Organism | Homo sapiens |
| Official full symbol | argonaute RISC component 1 |
| Gene type | protein-coding |
| Also known as | EIF2C, EIF2C1, GERP95, Q99, hAgo1 |
| Summary | This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| AGO1-201 | ENST00000373204.6 | 13712 | 857aa | Protein coding | CCDS398 | Q9UL18 | NM_012199.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| AGO1-204 | ENST00000674304.1 | 7478 | 857aa | Protein coding | CCDS398 | - | - | GENCODE basic, APPRIS P1, |
| AGO1-202 | ENST00000373206.5 | 3996 | 782aa | Protein coding | CCDS81300 | Q5TA58 | - | TSL:2, GENCODE basic, |
| AGO1-205 | ENST00000674426.1 | 13712 | 891aa | Protein coding | - | A0A6I8PTZ8 | - | GENCODE basic, |
| AGO1-203 | ENST00000635259.1 | 2836 | 133aa | Nonsense mediated decay | - | A0A0U1RQZ8 | - | CDS 5' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | AGO1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene AGO1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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