CRISPR-U™ Gene Knockout Cell Line Strategy

AGO1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human AGO1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol AGO1
Gene id 26523
Organism Homo sapiens
Official full symbol argonaute RISC component 1
Gene type protein-coding
Also known as EIF2C, EIF2C1, GERP95, Q99, hAgo1
Summary This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
AGO1-201 ENST00000373204.6 13712 857aa Protein coding CCDS398 Q9UL18 NM_012199.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
AGO1-204 ENST00000674304.1 7478 857aa Protein coding CCDS398 - - GENCODE basic, APPRIS P1,
AGO1-202 ENST00000373206.5 3996 782aa Protein coding CCDS81300 Q5TA58 - TSL:2, GENCODE basic,
AGO1-205 ENST00000674426.1 13712 891aa Protein coding - A0A6I8PTZ8 - GENCODE basic,
AGO1-203 ENST00000635259.1 2836 133aa Nonsense mediated decay - A0A0U1RQZ8 - CDS 5' incomplete, TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene AGO1
This KO Strategy loading
Red Cotton™ Notes Gene AGO1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×