CRISPR-U™ Gene Knockout Cell Line
Strategy
NARF Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NARF Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NARF |
| Gene id | 26502 |
| Organism | Homo sapiens |
| Official full symbol | nuclear prelamin A recognition factor |
| Gene type | protein-coding |
| Also known as | IOP2 |
| Summary | Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NARF-201 | ENST00000309794.16 | 3814 | 456aa | Protein coding | CCDS32777 | Q9UHQ1-1 | NM_012336.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| NARF-204 | ENST00000390006.8 | 1766 | 397aa | Protein coding | CCDS42404 | Q9UHQ1-4 | - | TSL:2, GENCODE basic, |
| NARF-202 | ENST00000345415.11 | 1487 | 408aa | Protein coding | CCDS42403 | Q9UHQ1-3 | - | TSL:1, GENCODE basic, |
| NARF-205 | ENST00000412079.6 | 1760 | 503aa | Protein coding | - | A0A088AWN8 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| NARF-206 | ENST00000457415.7 | 1668 | 502aa | Protein coding | - | E7EP87 | - | TSL:5, GENCODE basic, |
| NARF-207 | ENST00000577410.2 | 873 | 158aa | Protein coding | - | J3QRB0 | - | CDS 3' incomplete, TSL:5, |
| NARF-208 | ENST00000577432.5 | 866 | 262aa | Protein coding | - | J3KT43 | - | CDS 3' incomplete, TSL:5, |
| NARF-214 | ENST00000580296.5 | 733 | 86aa | Protein coding | - | J3QL28 | - | CDS 3' incomplete, TSL:3, |
| NARF-213 | ENST00000579198.5 | 599 | 37aa | Protein coding | - | J3KS03 | - | CDS 3' incomplete, TSL:5, |
| NARF-224 | ENST00000584411.5 | 525 | 87aa | Protein coding | - | J3QSD9 | - | CDS 3' incomplete, TSL:4, |
| NARF-226 | ENST00000584513.1 | 500 | 167aa | Protein coding | - | J3KTG5 | - | CDS 5' and 3' incomplete, TSL:3, |
| NARF-203 | ENST00000374611.9 | 1722 | 262aa | Nonsense mediated decay | - | E9PH27 | - | TSL:5, |
| NARF-220 | ENST00000582907.5 | 1454 | 188aa | Nonsense mediated decay | - | J3KRH7 | - | TSL:5, |
| NARF-218 | ENST00000581795.5 | 1430 | 143aa | Nonsense mediated decay | - | J3KTP5 | - | TSL:2, |
| NARF-216 | ENST00000581202.5 | 1013 | 217aa | Nonsense mediated decay | - | J3KS48 | - | TSL:5, |
| NARF-219 | ENST00000582585.1 | 891 | 99aa | Nonsense mediated decay | - | J3QLT6 | - | TSL:5, |
| NARF-215 | ENST00000580748.1 | 577 | 99aa | Nonsense mediated decay | - | J3QLT6 | - | TSL:5, |
| NARF-217 | ENST00000581743.5 | 785 | No protein | Processed transcript | - | - | - | TSL:5, |
| NARF-221 | ENST00000583181.5 | 505 | No protein | Processed transcript | - | - | - | TSL:5, |
| NARF-223 | ENST00000584192.5 | 495 | No protein | Processed transcript | - | - | - | TSL:3, |
| NARF-209 | ENST00000577812.5 | 2598 | No protein | Retained intron | - | - | - | TSL:5, |
| NARF-225 | ENST00000584445.5 | 2484 | No protein | Retained intron | - | - | - | TSL:2, |
| NARF-210 | ENST00000578082.5 | 841 | No protein | Retained intron | - | - | - | TSL:4, |
| NARF-211 | ENST00000578820.1 | 625 | No protein | Retained intron | - | - | - | TSL:3, |
| NARF-212 | ENST00000579083.1 | 561 | No protein | Retained intron | - | - | - | TSL:4, |
| NARF-222 | ENST00000583908.1 | 430 | No protein | Retained intron | - | - | - | TSL:3, |
| NARF-227 | ENST00000584965.1 | 300 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NARF |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NARF had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
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$599
Single gRNA lentivirus
Work flow
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