CRISPR-U™ Gene Knockout Cell Line Strategy

NARF Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NARF Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NARF
Gene id 26502
Organism Homo sapiens
Official full symbol nuclear prelamin A recognition factor
Gene type protein-coding
Also known as IOP2
Summary Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.
Genomic regions Chromosome 17
Strategy Summary
This gene has 11 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NARF-201 ENST00000309794.16 3814 456aa Protein coding CCDS32777 Q9UHQ1-1 NM_012336.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
NARF-204 ENST00000390006.8 1766 397aa Protein coding CCDS42404 Q9UHQ1-4 - TSL:2, GENCODE basic,
NARF-202 ENST00000345415.11 1487 408aa Protein coding CCDS42403 Q9UHQ1-3 - TSL:1, GENCODE basic,
NARF-205 ENST00000412079.6 1760 503aa Protein coding - A0A088AWN8 - TSL:5, GENCODE basic, APPRIS ALT2,
NARF-206 ENST00000457415.7 1668 502aa Protein coding - E7EP87 - TSL:5, GENCODE basic,
NARF-207 ENST00000577410.2 873 158aa Protein coding - J3QRB0 - CDS 3' incomplete, TSL:5,
NARF-208 ENST00000577432.5 866 262aa Protein coding - J3KT43 - CDS 3' incomplete, TSL:5,
NARF-214 ENST00000580296.5 733 86aa Protein coding - J3QL28 - CDS 3' incomplete, TSL:3,
NARF-213 ENST00000579198.5 599 37aa Protein coding - J3KS03 - CDS 3' incomplete, TSL:5,
NARF-224 ENST00000584411.5 525 87aa Protein coding - J3QSD9 - CDS 3' incomplete, TSL:4,
NARF-226 ENST00000584513.1 500 167aa Protein coding - J3KTG5 - CDS 5' and 3' incomplete, TSL:3,
NARF-203 ENST00000374611.9 1722 262aa Nonsense mediated decay - E9PH27 - TSL:5,
NARF-220 ENST00000582907.5 1454 188aa Nonsense mediated decay - J3KRH7 - TSL:5,
NARF-218 ENST00000581795.5 1430 143aa Nonsense mediated decay - J3KTP5 - TSL:2,
NARF-216 ENST00000581202.5 1013 217aa Nonsense mediated decay - J3KS48 - TSL:5,
NARF-219 ENST00000582585.1 891 99aa Nonsense mediated decay - J3QLT6 - TSL:5,
NARF-215 ENST00000580748.1 577 99aa Nonsense mediated decay - J3QLT6 - TSL:5,
NARF-217 ENST00000581743.5 785 No protein Processed transcript - - - TSL:5,
NARF-221 ENST00000583181.5 505 No protein Processed transcript - - - TSL:5,
NARF-223 ENST00000584192.5 495 No protein Processed transcript - - - TSL:3,
NARF-209 ENST00000577812.5 2598 No protein Retained intron - - - TSL:5,
NARF-225 ENST00000584445.5 2484 No protein Retained intron - - - TSL:2,
NARF-210 ENST00000578082.5 841 No protein Retained intron - - - TSL:4,
NARF-211 ENST00000578820.1 625 No protein Retained intron - - - TSL:3,
NARF-212 ENST00000579083.1 561 No protein Retained intron - - - TSL:4,
NARF-222 ENST00000583908.1 430 No protein Retained intron - - - TSL:3,
NARF-227 ENST00000584965.1 300 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NARF
This KO Strategy loading
Red Cotton™ Notes Gene NARF had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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