CRISPR-U™ Gene Knockout Cell Line Strategy

NUPR1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUPR1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NUPR1
Gene id 26471
Organism Homo sapiens
Official full symbol nuclear protein 1, transcriptional regulator
Gene type protein-coding
Also known as COM1, P8
Genomic regions Chromosome 16
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NUPR1-201 ENST00000324873.8 5291 82aa Protein coding CCDS10634 O60356-1 NM_012385.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NUPR1-202 ENST00000395641.2 550 100aa Protein coding CCDS42137 O60356-2 - TSL:2, GENCODE basic,
NUPR1-203 ENST00000567646.1 598 43aa Nonsense mediated decay - H3BS92 - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NUPR1
This KO Strategy loading
Red Cotton™ Notes Gene NUPR1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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