CRISPR-U™ Gene Knockout Cell Line
Strategy
NUPR1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUPR1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUPR1 |
| Gene id | 26471 |
| Organism | Homo sapiens |
| Official full symbol | nuclear protein 1, transcriptional regulator |
| Gene type | protein-coding |
| Also known as | COM1, P8 |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NUPR1-201 | ENST00000324873.8 | 5291 | 82aa | Protein coding | CCDS10634 | O60356-1 | NM_012385.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NUPR1-202 | ENST00000395641.2 | 550 | 100aa | Protein coding | CCDS42137 | O60356-2 | - | TSL:2, GENCODE basic, |
| NUPR1-203 | ENST00000567646.1 | 598 | 43aa | Nonsense mediated decay | - | H3BS92 | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUPR1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUPR1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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