CRISPR-U™ Gene Knockout Cell Line
Strategy
GCH1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GCH1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GCH1 |
| Gene id | 2643 |
| Organism | Homo sapiens |
| Official full symbol | GTP cyclohydrolase 1 |
| Gene type | protein-coding |
| Also known as | DYT14, DYT5, DYT5a, GCH, GTP-CH-1, GTPCH1, HPABH4B |
| Summary | This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GCH1-204 | ENST00000491895.7 | 2916 | 250aa | Protein coding | CCDS9720 | P30793-1 | NM_000161.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GCH1-202 | ENST00000395514.5 | 1977 | 250aa | Protein coding | CCDS9720 | P30793-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| GCH1-206 | ENST00000543643.6 | 1897 | 233aa | Protein coding | CCDS41954 | P30793-4 | - | TSL:1, GENCODE basic, |
| GCH1-205 | ENST00000536224.2 | 1805 | 213aa | Protein coding | CCDS45110 | P30793-2 | - | TSL:1, GENCODE basic, |
| GCH1-201 | ENST00000254299.8 | 2901 | No protein | Processed transcript | - | - | - | TSL:1, |
| GCH1-203 | ENST00000395521.6 | 351 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GCH1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GCH1 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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