CRISPR-U™ Gene Knockout Cell Line Strategy

GCH1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GCH1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GCH1
Gene id 2643
Organism Homo sapiens
Official full symbol GTP cyclohydrolase 1
Gene type protein-coding
Also known as DYT14, DYT5, DYT5a, GCH, GTP-CH-1, GTPCH1, HPABH4B
Summary This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme.
Genomic regions Chromosome 14
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GCH1-204 ENST00000491895.7 2916 250aa Protein coding CCDS9720 P30793-1 NM_000161.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GCH1-202 ENST00000395514.5 1977 250aa Protein coding CCDS9720 P30793-1 - TSL:1, GENCODE basic, APPRIS P1,
GCH1-206 ENST00000543643.6 1897 233aa Protein coding CCDS41954 P30793-4 - TSL:1, GENCODE basic,
GCH1-205 ENST00000536224.2 1805 213aa Protein coding CCDS45110 P30793-2 - TSL:1, GENCODE basic,
GCH1-201 ENST00000254299.8 2901 No protein Processed transcript - - - TSL:1,
GCH1-203 ENST00000395521.6 351 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GCH1
This KO Strategy loading
Red Cotton™ Notes Gene GCH1 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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