CRISPR-U™ Gene Knockout Cell Line Strategy

HSPB8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HSPB8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HSPB8
Gene id 26353
Organism Homo sapiens
Official full symbol heat shock protein family B (small) member 8
Gene type protein-coding
Also known as CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22
Summary The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.
Genomic regions Chromosome 12
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HSPB8-201 ENST00000281938.7 1861 196aa Protein coding CCDS9189 Q9UJY1 NM_014365.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HSPB8-204 ENST00000674542.1 1717 130aa Protein coding - - - GENCODE basic,
HSPB8-202 ENST00000541798.1 637 152aa Protein coding - H0YG30 - CDS 5' incomplete, TSL:3,
HSPB8-207 ENST00000674852.1 482 51aa Protein coding - - - CDS 5' incomplete,
HSPB8-206 ENST00000674763.1 296 98aa Protein coding - - - CDS 5' incomplete,
HSPB8-210 ENST00000675573.1 287 95aa Protein coding - - - CDS 5' incomplete,
HSPB8-209 ENST00000675211.1 354 29aa Nonsense mediated decay - - - CDS 5' incomplete,
HSPB8-208 ENST00000675110.1 337 28aa Nonsense mediated decay - - - CDS 5' incomplete,
HSPB8-205 ENST00000674715.1 1525 No protein Processed transcript - - - -
HSPB8-213 ENST00000676071.1 1084 No protein Processed transcript - - - -
HSPB8-211 ENST00000675900.1 928 No protein Processed transcript - - - -
HSPB8-203 ENST00000542496.1 419 No protein Processed transcript - - - TSL:2,
HSPB8-214 ENST00000676244.1 382 No protein Processed transcript - - - -
HSPB8-212 ENST00000676008.1 115 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HSPB8
This KO Strategy loading
Red Cotton™ Notes Gene HSPB8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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