CRISPR-U™ Gene Knockout Cell Line
Strategy
HSPB8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HSPB8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HSPB8 |
| Gene id | 26353 |
| Organism | Homo sapiens |
| Official full symbol | heat shock protein family B (small) member 8 |
| Gene type | protein-coding |
| Also known as | CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22 |
| Summary | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HSPB8-201 | ENST00000281938.7 | 1861 | 196aa | Protein coding | CCDS9189 | Q9UJY1 | NM_014365.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HSPB8-204 | ENST00000674542.1 | 1717 | 130aa | Protein coding | - | - | - | GENCODE basic, |
| HSPB8-202 | ENST00000541798.1 | 637 | 152aa | Protein coding | - | H0YG30 | - | CDS 5' incomplete, TSL:3, |
| HSPB8-207 | ENST00000674852.1 | 482 | 51aa | Protein coding | - | - | - | CDS 5' incomplete, |
| HSPB8-206 | ENST00000674763.1 | 296 | 98aa | Protein coding | - | - | - | CDS 5' incomplete, |
| HSPB8-210 | ENST00000675573.1 | 287 | 95aa | Protein coding | - | - | - | CDS 5' incomplete, |
| HSPB8-209 | ENST00000675211.1 | 354 | 29aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| HSPB8-208 | ENST00000675110.1 | 337 | 28aa | Nonsense mediated decay | - | - | - | CDS 5' incomplete, |
| HSPB8-205 | ENST00000674715.1 | 1525 | No protein | Processed transcript | - | - | - | - |
| HSPB8-213 | ENST00000676071.1 | 1084 | No protein | Processed transcript | - | - | - | - |
| HSPB8-211 | ENST00000675900.1 | 928 | No protein | Processed transcript | - | - | - | - |
| HSPB8-203 | ENST00000542496.1 | 419 | No protein | Processed transcript | - | - | - | TSL:2, |
| HSPB8-214 | ENST00000676244.1 | 382 | No protein | Processed transcript | - | - | - | - |
| HSPB8-212 | ENST00000676008.1 | 115 | No protein | Processed transcript | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HSPB8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HSPB8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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