CRISPR-U™ Gene Knockout Cell Line Strategy

GBP3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GBP3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GBP3
Gene id 2635
Organism Homo sapiens
Official full symbol guanylate binding protein 3
Gene type protein-coding
Summary This gene encodes a member of the guanylate-binding protein (GBP) family. GBPs specifically bind guanine nucleotides (GMP, GDP, and GTP) and contain two of the three consensus motifs found in typical GTP-binding proteins. The encoded protein interacts with a member of the germinal center kinase family. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GBP3-202 ENST00000370481.9 3037 595aa Protein coding CCDS717 Q9H0R5-1 NM_018284.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GBP3-205 ENST00000461384.6 702 218aa Protein coding - H3BNS1 - CDS 5' incomplete, TSL:2,
GBP3-210 ENST00000564665.1 599 123aa Protein coding - H3BPH2 - CDS 3' incomplete, TSL:4,
GBP3-204 ENST00000445969.1 411 137aa Protein coding - Q5T8M0 - CDS 5' and 3' incomplete, TSL:2,
GBP3-208 ENST00000493594.6 2561 91aa Nonsense mediated decay - H3BRX6 - TSL:1,
GBP3-207 ENST00000489444.6 2455 290aa Nonsense mediated decay - F6X827 - TSL:1,
GBP3-201 ENST00000235878.5 2062 142aa Nonsense mediated decay - F6SPX6 - TSL:2,
GBP3-211 ENST00000568006.1 583 96aa Nonsense mediated decay - H3BP53 - TSL:5,
GBP3-206 ENST00000475853.2 566 No protein Processed transcript - - - TSL:4,
GBP3-203 ENST00000370482.6 3678 No protein Retained intron - - - TSL:2,
GBP3-209 ENST00000564037.1 512 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GBP3
This KO Strategy loading
Red Cotton™ Notes Gene GBP3 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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