CRISPR-U™ Gene Knockout Cell Line
Strategy
GBP3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GBP3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GBP3 |
| Gene id | 2635 |
| Organism | Homo sapiens |
| Official full symbol | guanylate binding protein 3 |
| Gene type | protein-coding |
| Summary | This gene encodes a member of the guanylate-binding protein (GBP) family. GBPs specifically bind guanine nucleotides (GMP, GDP, and GTP) and contain two of the three consensus motifs found in typical GTP-binding proteins. The encoded protein interacts with a member of the germinal center kinase family. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GBP3-202 | ENST00000370481.9 | 3037 | 595aa | Protein coding | CCDS717 | Q9H0R5-1 | NM_018284.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GBP3-205 | ENST00000461384.6 | 702 | 218aa | Protein coding | - | H3BNS1 | - | CDS 5' incomplete, TSL:2, |
| GBP3-210 | ENST00000564665.1 | 599 | 123aa | Protein coding | - | H3BPH2 | - | CDS 3' incomplete, TSL:4, |
| GBP3-204 | ENST00000445969.1 | 411 | 137aa | Protein coding | - | Q5T8M0 | - | CDS 5' and 3' incomplete, TSL:2, |
| GBP3-208 | ENST00000493594.6 | 2561 | 91aa | Nonsense mediated decay | - | H3BRX6 | - | TSL:1, |
| GBP3-207 | ENST00000489444.6 | 2455 | 290aa | Nonsense mediated decay | - | F6X827 | - | TSL:1, |
| GBP3-201 | ENST00000235878.5 | 2062 | 142aa | Nonsense mediated decay | - | F6SPX6 | - | TSL:2, |
| GBP3-211 | ENST00000568006.1 | 583 | 96aa | Nonsense mediated decay | - | H3BP53 | - | TSL:5, |
| GBP3-206 | ENST00000475853.2 | 566 | No protein | Processed transcript | - | - | - | TSL:4, |
| GBP3-203 | ENST00000370482.6 | 3678 | No protein | Retained intron | - | - | - | TSL:2, |
| GBP3-209 | ENST00000564037.1 | 512 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GBP3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GBP3 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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