CRISPR-U™ Gene Knockout Cell Line
Strategy
GBP2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GBP2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GBP2 |
| Gene id | 2634 |
| Organism | Homo sapiens |
| Official full symbol | guanylate binding protein 2 |
| Gene type | protein-coding |
| Summary | This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GBP2-201 | ENST00000370466.4 | 4088 | 591aa | Protein coding | CCDS719 | P32456 | NM_004120.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GBP2-203 | ENST00000464839.5 | 3687 | 591aa | Nonsense mediated decay | CCDS719 | P32456 | - | TSL:2, |
| GBP2-204 | ENST00000493802.5 | 794 | No protein | Processed transcript | - | - | - | TSL:3, |
| GBP2-202 | ENST00000463660.1 | 3461 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GBP2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GBP2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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