CRISPR-U™ Gene Knockout Cell Line Strategy

GBP2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GBP2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GBP2
Gene id 2634
Organism Homo sapiens
Official full symbol guanylate binding protein 2
Gene type protein-coding
Summary This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas.
Genomic regions Chromosome 1
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GBP2-201 ENST00000370466.4 4088 591aa Protein coding CCDS719 P32456 NM_004120.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GBP2-203 ENST00000464839.5 3687 591aa Nonsense mediated decay CCDS719 P32456 - TSL:2,
GBP2-204 ENST00000493802.5 794 No protein Processed transcript - - - TSL:3,
GBP2-202 ENST00000463660.1 3461 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GBP2
This KO Strategy loading
Red Cotton™ Notes Gene GBP2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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