CRISPR-U™ Gene Knockout Cell Line Strategy

GATA4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GATA4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GATA4
Gene id 2626
Organism Homo sapiens
Official full symbol GATA binding protein 4
Gene type protein-coding
Also known as ASD2, TACHD, TOF, VSD1
Summary This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 8
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GATA4-207 ENST00000532059.6 3419 443aa Protein coding CCDS78303 P43694-2 NM_001308093.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
GATA4-201 ENST00000335135.8 3414 442aa Protein coding CCDS5983 P43694-1 - TSL:5, GENCODE basic, APPRIS ALT1,
GATA4-206 ENST00000528712.5 2614 236aa Protein coding CCDS78304 B3KUF4 - TSL:2, GENCODE basic,
GATA4-209 ENST00000622443.2 1982 441aa Protein coding - A0A087WZ09 - TSL:5, GENCODE basic,
GATA4-205 ENST00000528027.1 1010 116aa Protein coding - E9PRI5 - CDS 3' incomplete, TSL:2,
GATA4-208 ENST00000532977.1 877 16aa Protein coding - B6DU75 - CDS 3' incomplete, TSL:1,
GATA4-204 ENST00000526974.1 832 23aa Protein coding - R4GND5 - CDS 3' incomplete, TSL:2,
GATA4-203 ENST00000526716.5 686 175aa Protein coding - E9PKS4 - CDS 3' incomplete, TSL:4,
GATA4-202 ENST00000526021.1 1064 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GATA4
This KO Strategy loading
Red Cotton™ Notes Gene GATA4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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