CRISPR-U™ Gene Knockout Cell Line
Strategy
GATA4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GATA4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GATA4 |
| Gene id | 2626 |
| Organism | Homo sapiens |
| Official full symbol | GATA binding protein 4 |
| Gene type | protein-coding |
| Also known as | ASD2, TACHD, TOF, VSD1 |
| Summary | This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GATA4-207 | ENST00000532059.6 | 3419 | 443aa | Protein coding | CCDS78303 | P43694-2 | NM_001308093.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| GATA4-201 | ENST00000335135.8 | 3414 | 442aa | Protein coding | CCDS5983 | P43694-1 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| GATA4-206 | ENST00000528712.5 | 2614 | 236aa | Protein coding | CCDS78304 | B3KUF4 | - | TSL:2, GENCODE basic, |
| GATA4-209 | ENST00000622443.2 | 1982 | 441aa | Protein coding | - | A0A087WZ09 | - | TSL:5, GENCODE basic, |
| GATA4-205 | ENST00000528027.1 | 1010 | 116aa | Protein coding | - | E9PRI5 | - | CDS 3' incomplete, TSL:2, |
| GATA4-208 | ENST00000532977.1 | 877 | 16aa | Protein coding | - | B6DU75 | - | CDS 3' incomplete, TSL:1, |
| GATA4-204 | ENST00000526974.1 | 832 | 23aa | Protein coding | - | R4GND5 | - | CDS 3' incomplete, TSL:2, |
| GATA4-203 | ENST00000526716.5 | 686 | 175aa | Protein coding | - | E9PKS4 | - | CDS 3' incomplete, TSL:4, |
| GATA4-202 | ENST00000526021.1 | 1064 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GATA4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GATA4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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