CRISPR-U™ Gene Knockout Cell Line
Strategy
ULK3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ULK3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ULK3 |
| Gene id | 25989 |
| Organism | Homo sapiens |
| Official full symbol | unc-51 like kinase 3 |
| Gene type | protein-coding |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ULK3-220 | ENST00000569437.5 | 2611 | 470aa | Protein coding | CCDS76779 | Q6PHR2-3 | - | TSL:1, GENCODE basic, APPRIS P4, |
| ULK3-201 | ENST00000440863.7 | 2580 | 472aa | Protein coding | CCDS45305 | Q6PHR2-1 | NM_001099436.4 | TSL:2, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| ULK3-217 | ENST00000568667.5 | 1470 | 483aa | Protein coding | - | Q6PHR2-4 | - | TSL:5, GENCODE basic, |
| ULK3-223 | ENST00000631115.1 | 126 | 41aa | Protein coding | - | H3BP85 | - | TSL:5, GENCODE basic, |
| ULK3-224 | ENST00000647587.1 | 2674 | 41aa | Nonsense mediated decay | - | H3BP85 | - | - |
| ULK3-215 | ENST00000568210.5 | 1717 | 50aa | Nonsense mediated decay | - | H3BMW8 | - | TSL:2, |
| ULK3-216 | ENST00000568273.5 | 1711 | 50aa | Nonsense mediated decay | - | H3BMW8 | - | TSL:2, |
| ULK3-213 | ENST00000566631.5 | 1633 | 84aa | Nonsense mediated decay | - | H3BPN6 | - | TSL:2, |
| ULK3-205 | ENST00000563301.5 | 884 | 136aa | Nonsense mediated decay | - | H3BT78 | - | TSL:4, |
| ULK3-210 | ENST00000565790.5 | 567 | 42aa | Nonsense mediated decay | - | H3BTS3 | - | TSL:4, |
| ULK3-203 | ENST00000562161.5 | 561 | 61aa | Nonsense mediated decay | - | H3BS27 | - | TSL:4, |
| ULK3-209 | ENST00000565373.5 | 561 | 49aa | Nonsense mediated decay | - | H3BV76 | - | TSL:4, |
| ULK3-218 | ENST00000568679.1 | 539 | 41aa | Nonsense mediated decay | - | H3BP85 | - | TSL:5, |
| ULK3-222 | ENST00000570276.5 | 2951 | No protein | Retained intron | - | - | - | TSL:1, |
| ULK3-202 | ENST00000561725.5 | 2738 | No protein | Retained intron | - | - | - | TSL:5, |
| ULK3-212 | ENST00000566479.5 | 2715 | No protein | Retained intron | - | - | - | TSL:1, |
| ULK3-211 | ENST00000565881.5 | 1565 | No protein | Retained intron | - | - | - | TSL:5, |
| ULK3-204 | ENST00000562395.5 | 1004 | No protein | Retained intron | - | - | - | TSL:2, |
| ULK3-206 | ENST00000564029.5 | 942 | No protein | Retained intron | - | - | - | TSL:5, |
| ULK3-207 | ENST00000564767.5 | 916 | No protein | Retained intron | - | - | - | TSL:2, |
| ULK3-208 | ENST00000565011.5 | 886 | No protein | Retained intron | - | - | - | TSL:2, |
| ULK3-214 | ENST00000567472.5 | 841 | No protein | Retained intron | - | - | - | TSL:3, |
| ULK3-219 | ENST00000568718.5 | 707 | No protein | Retained intron | - | - | - | TSL:2, |
| ULK3-221 | ENST00000569813.5 | 579 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ULK3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ULK3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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