CRISPR-U™ Gene Knockout Cell Line
Strategy
C1orf43 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human C1orf43 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | C1orf43 |
| Gene id | 25912 |
| Organism | Homo sapiens |
| Official full symbol | chromosome 1 open reading frame 43 |
| Gene type | protein-coding |
| Also known as | HSPC012, NICE-3, NICE3, NS5ATP4, S863-3 |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| C1orf43-206 | ENST00000368521.10 | 1678 | 253aa | Protein coding | CCDS41404 | Q9BWL3-1 | NM_001098616.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| C1orf43-201 | ENST00000350592.7 | 1610 | 219aa | Protein coding | CCDS1061 | Q9BWL3-2 | - | TSL:1, GENCODE basic, |
| C1orf43-202 | ENST00000362076.8 | 1551 | 201aa | Protein coding | CCDS1062 | Q9BWL3-4 | - | TSL:1, GENCODE basic, |
| C1orf43-204 | ENST00000368518.5 | 1278 | 189aa | Protein coding | CCDS76220 | Q9BWL3-3 | - | TSL:1, GENCODE basic, |
| C1orf43-205 | ENST00000368519.5 | 1163 | 235aa | Protein coding | CCDS72924 | X6R6S3 | - | TSL:2, GENCODE basic, |
| C1orf43-203 | ENST00000368516.1 | 872 | 155aa | Protein coding | CCDS76219 | X6R6F3 | - | TSL:2, GENCODE basic, |
| C1orf43-210 | ENST00000640799.1 | 1287 | 170aa | Protein coding | - | A0A1W2PQP6 | - | TSL:5, GENCODE basic, |
| C1orf43-207 | ENST00000470180.2 | 1861 | 149aa | Nonsense mediated decay | - | A0A1W2PQS0 | - | TSL:5, |
| C1orf43-211 | ENST00000648267.1 | 1760 | 85aa | Nonsense mediated decay | - | A0A3B3IS96 | - | - |
| C1orf43-208 | ENST00000483282.1 | 456 | No protein | Processed transcript | - | - | - | TSL:3, |
| C1orf43-209 | ENST00000493814.1 | 441 | No protein | Processed transcript | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | C1orf43 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene C1orf43 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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