CRISPR-U™ Gene Knockout Cell Line Strategy

C1orf43 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human C1orf43 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol C1orf43
Gene id 25912
Organism Homo sapiens
Official full symbol chromosome 1 open reading frame 43
Gene type protein-coding
Also known as HSPC012, NICE-3, NICE3, NS5ATP4, S863-3
Genomic regions Chromosome 1
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
C1orf43-206 ENST00000368521.10 1678 253aa Protein coding CCDS41404 Q9BWL3-1 NM_001098616.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
C1orf43-201 ENST00000350592.7 1610 219aa Protein coding CCDS1061 Q9BWL3-2 - TSL:1, GENCODE basic,
C1orf43-202 ENST00000362076.8 1551 201aa Protein coding CCDS1062 Q9BWL3-4 - TSL:1, GENCODE basic,
C1orf43-204 ENST00000368518.5 1278 189aa Protein coding CCDS76220 Q9BWL3-3 - TSL:1, GENCODE basic,
C1orf43-205 ENST00000368519.5 1163 235aa Protein coding CCDS72924 X6R6S3 - TSL:2, GENCODE basic,
C1orf43-203 ENST00000368516.1 872 155aa Protein coding CCDS76219 X6R6F3 - TSL:2, GENCODE basic,
C1orf43-210 ENST00000640799.1 1287 170aa Protein coding - A0A1W2PQP6 - TSL:5, GENCODE basic,
C1orf43-207 ENST00000470180.2 1861 149aa Nonsense mediated decay - A0A1W2PQS0 - TSL:5,
C1orf43-211 ENST00000648267.1 1760 85aa Nonsense mediated decay - A0A3B3IS96 - -
C1orf43-208 ENST00000483282.1 456 No protein Processed transcript - - - TSL:3,
C1orf43-209 ENST00000493814.1 441 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene C1orf43
This KO Strategy loading
Red Cotton™ Notes Gene C1orf43 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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