CRISPR-U™ Gene Knockout Cell Line Strategy

MTHFD1L Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MTHFD1L Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MTHFD1L
Gene id 25902
Organism Homo sapiens
Official full symbol methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
Gene type protein-coding
Also known as FTHFSDC1, MTC1THFS, dJ292B18.2
Summary The protein encoded by this gene is involved in the synthesis of tetrahydrofolate (THF) in the mitochondrion. THF is important in the de novo synthesis of purines and thymidylate and in the regeneration of methionine from homocysteine. Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MTHFD1L-211 ENST00000611279.4 3475 979aa Protein coding CCDS75535 B7ZM99 - TSL:5, GENCODE basic, APPRIS ALT2,
MTHFD1L-203 ENST00000367321.8 3451 978aa Protein coding CCDS5228 Q6UB35-1 NM_015440.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
MTHFD1L-212 ENST00000618312.4 3159 913aa Protein coding CCDS75536 A0A087WVM4 - TSL:5, GENCODE basic, APPRIS ALT2,
MTHFD1L-201 ENST00000367307.8 1049 275aa Protein coding CCDS56457 Q6UB35-2 - TSL:1, GENCODE basic,
MTHFD1L-202 ENST00000367308.8 996 332aa Protein coding - H0Y327 - CDS 5' and 3' incomplete, TSL:5,
MTHFD1L-204 ENST00000420192.1 606 96aa Protein coding - Q4VXV2 - CDS 5' incomplete, TSL:2,
MTHFD1L-206 ENST00000423867.2 524 114aa Protein coding - Q5JYA3 - CDS 3' incomplete, TSL:2,
MTHFD1L-209 ENST00000453602.1 503 92aa Protein coding - Q4VXM0 - CDS 5' incomplete, TSL:5,
MTHFD1L-208 ENST00000450635.1 427 57aa Protein coding - Q4VXM1 - CDS 5' incomplete, TSL:5,
MTHFD1L-207 ENST00000441122.5 1134 108aa Nonsense mediated decay - Q5JYA8 - TSL:5,
MTHFD1L-210 ENST00000478643.1 421 No protein Processed transcript - - - TSL:3,
MTHFD1L-205 ENST00000421497.1 373 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MTHFD1L
This KO Strategy loading
Red Cotton™ Notes Gene MTHFD1L had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×