CRISPR-U™ Gene Knockout Cell Line Strategy

RCHY1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RCHY1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RCHY1
Gene id 25898
Organism Homo sapiens
Official full symbol ring finger and CHY zinc finger domain containing 1
Gene type protein-coding
Also known as ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363
Summary The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Genomic regions Chromosome 4
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RCHY1-201 ENST00000324439.9 4686 261aa Protein coding CCDS3567 Q96PM5-1 - TSL:1, GENCODE basic, APPRIS P1,
RCHY1-202 ENST00000380840.6 1735 221aa Protein coding CCDS63991 Q96PM5-7 - TSL:1, GENCODE basic,
RCHY1-208 ENST00000512706.5 1011 239aa Protein coding CCDS63992 Q96PM5-6 - TSL:3, GENCODE basic,
RCHY1-210 ENST00000513257.5 759 252aa Protein coding CCDS34012 Q96PM5-2 - TSL:1, GENCODE basic,
RCHY1-206 ENST00000507014.1 650 212aa Protein coding CCDS63990 Q96PM5-8 - TSL:3, GENCODE basic,
RCHY1-204 ENST00000505105.5 1304 188aa Nonsense mediated decay - Q96PM5-4 - TSL:1,
RCHY1-203 ENST00000504085.5 1123 107aa Nonsense mediated decay - D6RAF6 - TSL:1,
RCHY1-211 ENST00000513909.5 728 111aa Nonsense mediated decay - H0YAI4 - CDS 5' incomplete, TSL:3,
RCHY1-212 ENST00000514021.1 436 No protein Processed transcript - - - TSL:3,
RCHY1-213 ENST00000514589.5 2443 No protein Retained intron - - - TSL:2,
RCHY1-207 ENST00000512567.5 1164 No protein Retained intron - - - TSL:1,
RCHY1-205 ENST00000505514.5 1089 No protein Retained intron - - - TSL:5,
RCHY1-209 ENST00000513083.5 540 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RCHY1
This KO Strategy loading
Red Cotton™ Notes Gene RCHY1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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