CRISPR-U™ Gene Knockout Cell Line
Strategy
RCHY1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RCHY1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RCHY1 |
| Gene id | 25898 |
| Organism | Homo sapiens |
| Official full symbol | ring finger and CHY zinc finger domain containing 1 |
| Gene type | protein-coding |
| Also known as | ARNIP, CHIMP, PIRH2, PRO1996, RNF199, ZCHY, ZNF363 |
| Summary | The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Genomic regions | Chromosome 4 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RCHY1-201 | ENST00000324439.9 | 4686 | 261aa | Protein coding | CCDS3567 | Q96PM5-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| RCHY1-202 | ENST00000380840.6 | 1735 | 221aa | Protein coding | CCDS63991 | Q96PM5-7 | - | TSL:1, GENCODE basic, |
| RCHY1-208 | ENST00000512706.5 | 1011 | 239aa | Protein coding | CCDS63992 | Q96PM5-6 | - | TSL:3, GENCODE basic, |
| RCHY1-210 | ENST00000513257.5 | 759 | 252aa | Protein coding | CCDS34012 | Q96PM5-2 | - | TSL:1, GENCODE basic, |
| RCHY1-206 | ENST00000507014.1 | 650 | 212aa | Protein coding | CCDS63990 | Q96PM5-8 | - | TSL:3, GENCODE basic, |
| RCHY1-204 | ENST00000505105.5 | 1304 | 188aa | Nonsense mediated decay | - | Q96PM5-4 | - | TSL:1, |
| RCHY1-203 | ENST00000504085.5 | 1123 | 107aa | Nonsense mediated decay | - | D6RAF6 | - | TSL:1, |
| RCHY1-211 | ENST00000513909.5 | 728 | 111aa | Nonsense mediated decay | - | H0YAI4 | - | CDS 5' incomplete, TSL:3, |
| RCHY1-212 | ENST00000514021.1 | 436 | No protein | Processed transcript | - | - | - | TSL:3, |
| RCHY1-213 | ENST00000514589.5 | 2443 | No protein | Retained intron | - | - | - | TSL:2, |
| RCHY1-207 | ENST00000512567.5 | 1164 | No protein | Retained intron | - | - | - | TSL:1, |
| RCHY1-205 | ENST00000505514.5 | 1089 | No protein | Retained intron | - | - | - | TSL:5, |
| RCHY1-209 | ENST00000513083.5 | 540 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RCHY1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RCHY1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: