CRISPR-U™ Gene Knockout Cell Line
Strategy
DCAF12 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DCAF12 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DCAF12 |
| Gene id | 25853 |
| Organism | Homo sapiens |
| Official full symbol | DDB1 and CUL4 associated factor 12 |
| Gene type | protein-coding |
| Also known as | CT102, KIAA1892, TCC52, WDR40A |
| Summary | This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DCAF12-201 | ENST00000361264.9 | 3592 | 453aa | Protein coding | CCDS6549 | Q5T6F0 | NM_015397.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DCAF12-202 | ENST00000396990.6 | 803 | 227aa | Protein coding | - | X6RBJ8 | - | CDS 3' incomplete, TSL:3, |
| DCAF12-203 | ENST00000450964.1 | 676 | 189aa | Protein coding | - | X6RL26 | - | CDS 3' incomplete, TSL:5, |
| DCAF12-204 | ENST00000463286.1 | 682 | No protein | Processed transcript | - | - | - | TSL:3, |
| DCAF12-205 | ENST00000466402.1 | 673 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DCAF12 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DCAF12 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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