CRISPR-U™ Gene Knockout Cell Line Strategy

DCAF12 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DCAF12 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DCAF12
Gene id 25853
Organism Homo sapiens
Official full symbol DDB1 and CUL4 associated factor 12
Gene type protein-coding
Also known as CT102, KIAA1892, TCC52, WDR40A
Summary This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates.
Genomic regions Chromosome 9
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DCAF12-201 ENST00000361264.9 3592 453aa Protein coding CCDS6549 Q5T6F0 NM_015397.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DCAF12-202 ENST00000396990.6 803 227aa Protein coding - X6RBJ8 - CDS 3' incomplete, TSL:3,
DCAF12-203 ENST00000450964.1 676 189aa Protein coding - X6RL26 - CDS 3' incomplete, TSL:5,
DCAF12-204 ENST00000463286.1 682 No protein Processed transcript - - - TSL:3,
DCAF12-205 ENST00000466402.1 673 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DCAF12
This KO Strategy loading
Red Cotton™ Notes Gene DCAF12 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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