CRISPR-U™ Gene Knockout Cell Line
Strategy
METTL7A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human METTL7A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | METTL7A |
| Gene id | 25840 |
| Organism | Homo sapiens |
| Official full symbol | methyltransferase like 7A |
| Gene type | protein-coding |
| Also known as | AAM-B, AAMB |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| METTL7A-203 | ENST00000548553.1 | 4076 | 244aa | Protein coding | CCDS8804 | A0A024R118 Q9H8H3 | - | TSL:5, GENCODE basic, APPRIS P1, |
| METTL7A-201 | ENST00000332160.5 | 3117 | 244aa | Protein coding | CCDS8804 | A0A024R118 Q9H8H3 | NM_014033.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| METTL7A-205 | ENST00000550502.1 | 618 | 180aa | Protein coding | - | F8VQX6 | - | CDS 3' incomplete, TSL:5, |
| METTL7A-202 | ENST00000547104.1 | 1825 | 244aa | Nonsense mediated decay | CCDS8804 | A0A024R118 Q9H8H3 | - | TSL:1, |
| METTL7A-204 | ENST00000550097.1 | 1846 | 244aa | Nonsense mediated decay | - | H0YI09 | - | CDS 5' incomplete, TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | METTL7A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene METTL7A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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