CRISPR-U™ Gene Knockout Cell Line Strategy

GALK1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GALK1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GALK1
Gene id 2584
Organism Homo sapiens
Official full symbol galactokinase 1
Gene type protein-coding
Also known as GALK, GK1, HEL-S-19
Summary Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population.
Genomic regions Chromosome 17
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GALK1-201 ENST00000225614.6 1445 392aa Protein coding CCDS11728 P51570-1 - TSL:2, GENCODE basic, APPRIS P1,
GALK1-205 ENST00000588479.6 1397 392aa Protein coding CCDS11728 P51570-1 NM_000154.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GALK1-209 ENST00000592997.5 717 184aa Protein coding - K7EII7 - CDS 5' incomplete, TSL:2,
GALK1-202 ENST00000586244.1 1180 120aa Nonsense mediated decay - K7ERJ9 - TSL:1,
GALK1-204 ENST00000587707.1 667 91aa Nonsense mediated decay - K7ERN9 - CDS 5' incomplete, TSL:5,
GALK1-208 ENST00000592494.1 1121 No protein Retained intron - - - TSL:5,
GALK1-206 ENST00000589030.1 866 No protein Retained intron - - - TSL:2,
GALK1-203 ENST00000586733.1 465 No protein Retained intron - - - TSL:2,
GALK1-207 ENST00000589643.1 313 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GALK1
This KO Strategy loading
Red Cotton™ Notes Gene GALK1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×