CRISPR-U™ Gene Knockout Cell Line
Strategy
GALK1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GALK1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GALK1 |
| Gene id | 2584 |
| Organism | Homo sapiens |
| Official full symbol | galactokinase 1 |
| Gene type | protein-coding |
| Also known as | GALK, GK1, HEL-S-19 |
| Summary | Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GALK1-201 | ENST00000225614.6 | 1445 | 392aa | Protein coding | CCDS11728 | P51570-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| GALK1-205 | ENST00000588479.6 | 1397 | 392aa | Protein coding | CCDS11728 | P51570-1 | NM_000154.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GALK1-209 | ENST00000592997.5 | 717 | 184aa | Protein coding | - | K7EII7 | - | CDS 5' incomplete, TSL:2, |
| GALK1-202 | ENST00000586244.1 | 1180 | 120aa | Nonsense mediated decay | - | K7ERJ9 | - | TSL:1, |
| GALK1-204 | ENST00000587707.1 | 667 | 91aa | Nonsense mediated decay | - | K7ERN9 | - | CDS 5' incomplete, TSL:5, |
| GALK1-208 | ENST00000592494.1 | 1121 | No protein | Retained intron | - | - | - | TSL:5, |
| GALK1-206 | ENST00000589030.1 | 866 | No protein | Retained intron | - | - | - | TSL:2, |
| GALK1-203 | ENST00000586733.1 | 465 | No protein | Retained intron | - | - | - | TSL:2, |
| GALK1-207 | ENST00000589643.1 | 313 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GALK1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GALK1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: