CRISPR-U™ Gene Knockout Cell Line
Strategy
DSTYK Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DSTYK Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DSTYK |
| Gene id | 25778 |
| Organism | Homo sapiens |
| Official full symbol | dual serine/threonine and tyrosine protein kinase |
| Gene type | protein-coding |
| Also known as | CAKUT1, DustyPK, HDCMD38P, RIP5, RIPK5, SPG23 |
| Summary | This gene encodes a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. It is thought to function as a regulator of cell death. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DSTYK-202 | ENST00000367162.8 | 8010 | 929aa | Protein coding | CCDS1451 | Q6XUX3-1 | NM_015375.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DSTYK-201 | ENST00000367161.7 | 7739 | 884aa | Protein coding | CCDS1452 | Q6XUX3-2 | - | TSL:1, GENCODE basic, |
| DSTYK-203 | ENST00000615388.1 | 1042 | 179aa | Protein coding | - | Q6XUX3-4 | - | TSL:5, GENCODE basic, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DSTYK |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DSTYK had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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