CRISPR-U™ Gene Knockout Cell Line Strategy

PCSK9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PCSK9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PCSK9
Gene id 255738
Organism Homo sapiens
Official full symbol proprotein convertase subtilisin/kexin type 9
Gene type protein-coding
Also known as FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9
Summary This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PCSK9-201 ENST00000302118.5 3637 692aa Protein coding CCDS603 Q8NBP7-1 NM_174936.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PCSK9-205 ENST00000673903.1 3194 567aa Protein coding - A0A669KBG0 - GENCODE basic,
PCSK9-204 ENST00000673726.1 1204 204aa Nonsense mediated decay - A0A669KAY4 - -
PCSK9-206 ENST00000673913.1 873 169aa Nonsense mediated decay - A0A669KB81 - CDS 5' incomplete,
PCSK9-203 ENST00000673662.1 603 No protein Processed transcript - - - -
PCSK9-202 ENST00000490692.1 3891 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PCSK9
This KO Strategy loading
Red Cotton™ Notes Gene PCSK9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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