CRISPR-U™ Gene Knockout Cell Line Strategy

IFI6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFI6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFI6
Gene id 2537
Organism Homo sapiens
Official full symbol interferon alpha inducible protein 6
Gene type protein-coding
Also known as 6-16, FAM14C, G1P3, IFI-6-16, IFI616
Summary This gene was first identified as one of the many genes induced by interferon. The encoded protein may play a critical role in the regulation of apoptosis. A minisatellite that consists of 26 repeats of a 12 nucleotide repeating element resembling the mammalian splice donor consensus sequence begins near the end of the second exon. Alternatively spliced transcript variants that encode different isoforms by using the two downstream repeat units as splice donor sites have been described.
Genomic regions Chromosome 1
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFI6-204 ENST00000679644.1 911 130aa Protein coding CCDS306 A0A348GSI1 - GENCODE basic, APPRIS ALT2,
IFI6-203 ENST00000362020.4 828 134aa Protein coding CCDS307 P09912-2 - TSL:1, GENCODE basic, APPRIS P4,
IFI6-201 ENST00000339145.8 822 138aa Protein coding CCDS308 P09912-3 - TSL:2, GENCODE basic, APPRIS ALT2,
IFI6-202 ENST00000361157.11 812 130aa Protein coding CCDS306 P09912-1 NM_002038.4 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFI6
This KO Strategy loading
Red Cotton™ Notes Gene IFI6 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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