CRISPR-U™ Gene Knockout Cell Line Strategy

MTOR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MTOR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MTOR
Gene id 2475
Organism Homo sapiens
Official full symbol mechanistic target of rapamycin kinase
Gene type protein-coding
Also known as FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
Summary The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MTOR-201 ENST00000361445.9 8721 2549aa Protein coding CCDS127 P42345 NM_004958.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MTOR-202 ENST00000376838.5 4017 754aa Protein coding - B1AKP8 - TSL:2, GENCODE basic,
MTOR-203 ENST00000455339.1 694 161aa Protein coding - B1AKQ2 - CDS 3' incomplete, TSL:3,
MTOR-207 ENST00000495435.1 2356 No protein Processed transcript - - - TSL:4,
MTOR-206 ENST00000490931.1 1111 No protein Processed transcript - - - TSL:5,
MTOR-204 ENST00000473471.5 862 No protein Processed transcript - - - TSL:3,
MTOR-205 ENST00000476768.1 610 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MTOR
This KO Strategy loading
Red Cotton™ Notes Gene MTOR had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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