CRISPR-U™ Gene Knockout Cell Line
Strategy
MTOR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MTOR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MTOR |
| Gene id | 2475 |
| Organism | Homo sapiens |
| Official full symbol | mechanistic target of rapamycin kinase |
| Gene type | protein-coding |
| Also known as | FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS |
| Summary | The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MTOR-201 | ENST00000361445.9 | 8721 | 2549aa | Protein coding | CCDS127 | P42345 | NM_004958.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MTOR-202 | ENST00000376838.5 | 4017 | 754aa | Protein coding | - | B1AKP8 | - | TSL:2, GENCODE basic, |
| MTOR-203 | ENST00000455339.1 | 694 | 161aa | Protein coding | - | B1AKQ2 | - | CDS 3' incomplete, TSL:3, |
| MTOR-207 | ENST00000495435.1 | 2356 | No protein | Processed transcript | - | - | - | TSL:4, |
| MTOR-206 | ENST00000490931.1 | 1111 | No protein | Processed transcript | - | - | - | TSL:5, |
| MTOR-204 | ENST00000473471.5 | 862 | No protein | Processed transcript | - | - | - | TSL:3, |
| MTOR-205 | ENST00000476768.1 | 610 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MTOR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MTOR had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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