CRISPR-U™ Gene Knockout Cell Line Strategy

IFIT5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IFIT5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IFIT5
Gene id 24138
Organism Homo sapiens
Official full symbol interferon induced protein with tetratricopeptide repeats 5
Gene type protein-coding
Also known as ISG58, P58, RI58
Genomic regions Chromosome 10
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IFIT5-201 ENST00000371795.5 4029 482aa Protein coding CCDS7403 Q13325-1 NM_012420.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IFIT5-208 ENST00000681422.1 4046 376aa Protein coding - - - GENCODE basic,
IFIT5-202 ENST00000679632.1 3281 136aa Protein coding - - - CDS 5' incomplete,
IFIT5-206 ENST00000681348.1 1513 434aa Protein coding - - - GENCODE basic,
IFIT5-204 ENST00000681163.1 524 118aa Protein coding - - - GENCODE basic,
IFIT5-203 ENST00000680591.1 451 120aa Protein coding - - - CDS 3' incomplete,
IFIT5-207 ENST00000681359.1 266 No protein Processed transcript - - - -
IFIT5-205 ENST00000681270.1 60 No protein Processed transcript - - - -
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IFIT5
This KO Strategy loading
Red Cotton™ Notes Gene IFIT5 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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