CRISPR-U™ Gene Knockout Cell Line
Strategy
IFIT5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IFIT5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IFIT5 |
| Gene id | 24138 |
| Organism | Homo sapiens |
| Official full symbol | interferon induced protein with tetratricopeptide repeats 5 |
| Gene type | protein-coding |
| Also known as | ISG58, P58, RI58 |
| Genomic regions | Chromosome 10 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IFIT5-201 | ENST00000371795.5 | 4029 | 482aa | Protein coding | CCDS7403 | Q13325-1 | NM_012420.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IFIT5-208 | ENST00000681422.1 | 4046 | 376aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT5-202 | ENST00000679632.1 | 3281 | 136aa | Protein coding | - | - | - | CDS 5' incomplete, |
| IFIT5-206 | ENST00000681348.1 | 1513 | 434aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT5-204 | ENST00000681163.1 | 524 | 118aa | Protein coding | - | - | - | GENCODE basic, |
| IFIT5-203 | ENST00000680591.1 | 451 | 120aa | Protein coding | - | - | - | CDS 3' incomplete, |
| IFIT5-207 | ENST00000681359.1 | 266 | No protein | Processed transcript | - | - | - | - |
| IFIT5-205 | ENST00000681270.1 | 60 | No protein | Processed transcript | - | - | - | - |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IFIT5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IFIT5 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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