CRISPR-U™ Gene Knockout Cell Line
Strategy
MTCH1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MTCH1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MTCH1 |
| Gene id | 23787 |
| Organism | Homo sapiens |
| Official full symbol | mitochondrial carrier 1 |
| Gene type | protein-coding |
| Also known as | CGI-64, PIG60, PSAP, SLC25A49 |
| Summary | This gene encodes a member of the mitochondrial carrier family. The encoded protein is localized to the mitochondrion inner membrane and induces apoptosis independent of the proapoptotic proteins Bax and Bak. Pseudogenes on chromosomes 6 and 11 have been identified for this gene. Alternatively spliced transcript variants encoding multiple isoforms have been observed. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MTCH1-201 | ENST00000373616.9 | 2006 | 372aa | Protein coding | CCDS4828 | Q9NZJ7-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| MTCH1-202 | ENST00000373627.10 | 1955 | 389aa | Protein coding | CCDS64411 | Q9NZJ7-1 | NM_001271641.2 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| MTCH1-204 | ENST00000460219.2 | 1572 | 394aa | Protein coding | - | H0Y8C3 | - | CDS 5' incomplete, TSL:2, |
| MTCH1-203 | ENST00000418541.6 | 602 | 201aa | Protein coding | - | H7C196 | - | CDS 5' and 3' incomplete, TSL:3, |
| MTCH1-205 | ENST00000471737.1 | 629 | No protein | Processed transcript | - | - | - | TSL:2, |
| MTCH1-206 | ENST00000492754.1 | 373 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MTCH1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MTCH1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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