CRISPR-U™ Gene Knockout Cell Line Strategy

APOL2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human APOL2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol APOL2
Gene id 23780
Organism Homo sapiens
Official full symbol apolipoprotein L2
Gene type protein-coding
Also known as APOL-II, APOL3
Summary This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. Two transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 22
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
APOL2-201 ENST00000249066.10 2685 337aa Protein coding CCDS43014 A0A024R1M8 Q9BQE5 - TSL:1, GENCODE basic, APPRIS P2,
APOL2-202 ENST00000358502.10 2429 337aa Protein coding CCDS43014 A0A024R1M8 Q9BQE5 NM_030882.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
APOL2-203 ENST00000451256.6 1421 449aa Protein coding - J3KQL8 - TSL:2, GENCODE basic, APPRIS ALT2,
APOL2-209 ENST00000529194.5 611 128aa Protein coding - E9PM95 - CDS 3' incomplete, TSL:3,
APOL2-204 ENST00000454728.5 577 74aa Protein coding - B0QYK8 - CDS 3' incomplete, TSL:4,
APOL2-206 ENST00000476579.1 583 No protein Processed transcript - - - TSL:4,
APOL2-205 ENST00000473549.1 579 No protein Processed transcript - - - TSL:4,
APOL2-208 ENST00000489186.1 579 No protein Processed transcript - - - TSL:3,
APOL2-207 ENST00000484830.1 527 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene APOL2
This KO Strategy loading
Red Cotton™ Notes Gene APOL2 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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