CRISPR-U™ Gene Knockout Cell Line
Strategy
APOL2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human APOL2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | APOL2 |
| Gene id | 23780 |
| Organism | Homo sapiens |
| Official full symbol | apolipoprotein L2 |
| Gene type | protein-coding |
| Also known as | APOL-II, APOL3 |
| Summary | This gene is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. Two transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| APOL2-201 | ENST00000249066.10 | 2685 | 337aa | Protein coding | CCDS43014 | A0A024R1M8 Q9BQE5 | - | TSL:1, GENCODE basic, APPRIS P2, |
| APOL2-202 | ENST00000358502.10 | 2429 | 337aa | Protein coding | CCDS43014 | A0A024R1M8 Q9BQE5 | NM_030882.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| APOL2-203 | ENST00000451256.6 | 1421 | 449aa | Protein coding | - | J3KQL8 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| APOL2-209 | ENST00000529194.5 | 611 | 128aa | Protein coding | - | E9PM95 | - | CDS 3' incomplete, TSL:3, |
| APOL2-204 | ENST00000454728.5 | 577 | 74aa | Protein coding | - | B0QYK8 | - | CDS 3' incomplete, TSL:4, |
| APOL2-206 | ENST00000476579.1 | 583 | No protein | Processed transcript | - | - | - | TSL:4, |
| APOL2-205 | ENST00000473549.1 | 579 | No protein | Processed transcript | - | - | - | TSL:4, |
| APOL2-208 | ENST00000489186.1 | 579 | No protein | Processed transcript | - | - | - | TSL:3, |
| APOL2-207 | ENST00000484830.1 | 527 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | APOL2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene APOL2 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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