CRISPR-U™ Gene Knockout Cell Line Strategy

PITPNB Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PITPNB Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PITPNB
Gene id 23760
Organism Homo sapiens
Official full symbol phosphatidylinositol transfer protein beta
Gene type protein-coding
Also known as PI-TP-beta, PtdInsTP, VIB1B
Summary This gene encodes a cytoplasmic protein that catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes. This transfer activity is required for COPI complex-mediated retrograde transport from the Golgi apparatus to the endoplasmic reticulum. Alternative splicing of this gene results in multiple transcript variants.
Genomic regions Chromosome 22
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PITPNB-202 ENST00000335272.10 2914 271aa Protein coding CCDS13842 P48739-1 NM_012399.5 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
PITPNB-201 ENST00000320996.14 2826 272aa Protein coding CCDS63433 P48739-2 - TSL:1, GENCODE basic, APPRIS ALT1,
PITPNB-211 ENST00000634017.1 1017 273aa Protein coding CCDS63432 P48739-3 - TSL:2, GENCODE basic,
PITPNB-205 ENST00000455418.7 2963 271aa Protein coding - A0A0A0MSW4 - TSL:5, GENCODE basic, APPRIS ALT1,
PITPNB-203 ENST00000415296.5 884 120aa Protein coding - B3KYB6 - CDS 3' incomplete, TSL:3,
PITPNB-204 ENST00000436663.1 590 167aa Protein coding - B3KYB7 - CDS 3' incomplete, TSL:2,
PITPNB-209 ENST00000477861.1 786 No protein Retained intron - - - TSL:5,
PITPNB-208 ENST00000471825.1 766 No protein Retained intron - - - TSL:2,
PITPNB-207 ENST00000465179.1 629 No protein Retained intron - - - TSL:2,
PITPNB-206 ENST00000460566.5 592 No protein Retained intron - - - TSL:4,
PITPNB-210 ENST00000493007.1 564 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PITPNB
This KO Strategy loading
Red Cotton™ Notes Gene PITPNB had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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