CRISPR-U™ Gene Knockout Cell Line
Strategy
PRKD3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRKD3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRKD3 |
| Gene id | 23683 |
| Organism | Homo sapiens |
| Official full symbol | protein kinase D3 |
| Gene type | protein-coding |
| Also known as | EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU |
| Summary | This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRKD3-201 | ENST00000234179.8 | 6156 | 890aa | Protein coding | CCDS1789 | O94806-1 | NM_005813.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRKD3-202 | ENST00000379066.5 | 6111 | 890aa | Protein coding | CCDS1789 | O94806-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PRKD3-203 | ENST00000443187.1 | 695 | 181aa | Protein coding | - | C9JKP8 | - | CDS 3' incomplete, TSL:4, |
| PRKD3-205 | ENST00000452104.5 | 555 | 185aa | Protein coding | - | H0Y5M6 | - | CDS 5' and 3' incomplete, TSL:4, |
| PRKD3-204 | ENST00000443977.1 | 518 | 172aa | Protein coding | - | H7C172 | - | CDS 5' and 3' incomplete, TSL:3, |
| PRKD3-209 | ENST00000477132.1 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
| PRKD3-210 | ENST00000494667.5 | 4326 | No protein | Retained intron | - | - | - | TSL:1, |
| PRKD3-207 | ENST00000469275.5 | 2642 | No protein | Retained intron | - | - | - | TSL:2, |
| PRKD3-208 | ENST00000475912.1 | 760 | No protein | Retained intron | - | - | - | TSL:3, |
| PRKD3-206 | ENST00000464552.1 | 436 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRKD3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRKD3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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