CRISPR-U™ Gene Knockout Cell Line Strategy

PRKD3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRKD3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRKD3
Gene id 23683
Organism Homo sapiens
Official full symbol protein kinase D3
Gene type protein-coding
Also known as EPK2, PKC-NU, PKD3, PRKCN, nPKC-NU
Summary This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking.
Genomic regions Chromosome 2
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRKD3-201 ENST00000234179.8 6156 890aa Protein coding CCDS1789 O94806-1 NM_005813.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRKD3-202 ENST00000379066.5 6111 890aa Protein coding CCDS1789 O94806-1 - TSL:5, GENCODE basic, APPRIS P1,
PRKD3-203 ENST00000443187.1 695 181aa Protein coding - C9JKP8 - CDS 3' incomplete, TSL:4,
PRKD3-205 ENST00000452104.5 555 185aa Protein coding - H0Y5M6 - CDS 5' and 3' incomplete, TSL:4,
PRKD3-204 ENST00000443977.1 518 172aa Protein coding - H7C172 - CDS 5' and 3' incomplete, TSL:3,
PRKD3-209 ENST00000477132.1 539 No protein Processed transcript - - - TSL:4,
PRKD3-210 ENST00000494667.5 4326 No protein Retained intron - - - TSL:1,
PRKD3-207 ENST00000469275.5 2642 No protein Retained intron - - - TSL:2,
PRKD3-208 ENST00000475912.1 760 No protein Retained intron - - - TSL:3,
PRKD3-206 ENST00000464552.1 436 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRKD3
This KO Strategy loading
Red Cotton™ Notes Gene PRKD3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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