CRISPR-U™ Gene Knockout Cell Line
Strategy
TRIM29 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TRIM29 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TRIM29 |
| Gene id | 23650 |
| Organism | Homo sapiens |
| Official full symbol | tripartite motif containing 29 |
| Gene type | protein-coding |
| Also known as | ATDC |
| Summary | The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TRIM29-201 | ENST00000341846.10 | 2977 | 588aa | Protein coding | CCDS8428 | Q14134-1 | NM_012101.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TRIM29-213 | ENST00000529044.5 | 1233 | 327aa | Protein coding | CCDS81637 | E9PRL4 | - | TSL:2, GENCODE basic, |
| TRIM29-210 | ENST00000528870.5 | 2217 | 121aa | Protein coding | - | E9PIQ2 | - | TSL:2, GENCODE basic, |
| TRIM29-219 | ENST00000532833.1 | 555 | 89aa | Protein coding | - | E9PI31 | - | CDS 3' incomplete, TSL:4, |
| TRIM29-214 | ENST00000529495.1 | 551 | 95aa | Protein coding | - | E9PLI4 | - | CDS 3' incomplete, TSL:5, |
| TRIM29-212 | ENST00000529040.1 | 549 | 38aa | Protein coding | - | E9PM74 | - | CDS 3' incomplete, TSL:4, |
| TRIM29-211 | ENST00000529011.1 | 547 | 97aa | Protein coding | - | E9PJ94 | - | CDS 3' incomplete, TSL:4, |
| TRIM29-220 | ENST00000533302.5 | 514 | 169aa | Protein coding | - | H0YF27 | - | CDS 5' incomplete, TSL:4, |
| TRIM29-221 | ENST00000627238.1 | 510 | 169aa | Protein coding | - | E9PJD4 | - | TSL:5, GENCODE basic, |
| TRIM29-209 | ENST00000526881.1 | 385 | 94aa | Protein coding | - | E9PPX1 | - | CDS 3' incomplete, TSL:3, |
| TRIM29-202 | ENST00000475051.4 | 1702 | 169aa | Nonsense mediated decay | - | E9PJD4 | - | TSL:5, |
| TRIM29-217 | ENST00000531555.5 | 535 | 119aa | Nonsense mediated decay | - | H0YD78 | - | CDS 5' incomplete, TSL:3, |
| TRIM29-204 | ENST00000524816.7 | 1632 | No protein | Processed transcript | - | - | - | TSL:5, |
| TRIM29-207 | ENST00000525887.5 | 965 | No protein | Processed transcript | - | - | - | TSL:2, |
| TRIM29-206 | ENST00000525327.5 | 548 | No protein | Processed transcript | - | - | - | TSL:3, |
| TRIM29-205 | ENST00000524956.4 | 314 | No protein | Processed transcript | - | - | - | TSL:5, |
| TRIM29-203 | ENST00000524664.5 | 285 | No protein | Processed transcript | - | - | - | TSL:2, |
| TRIM29-208 | ENST00000526161.5 | 1836 | No protein | Retained intron | - | - | - | TSL:1, |
| TRIM29-216 | ENST00000530919.5 | 1227 | No protein | Retained intron | - | - | - | TSL:3, |
| TRIM29-215 | ENST00000530470.1 | 771 | No protein | Retained intron | - | - | - | TSL:2, |
| TRIM29-218 | ENST00000532195.1 | 673 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TRIM29 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TRIM29 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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