CRISPR-U™ Gene Knockout Cell Line Strategy

TRIM29 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TRIM29 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TRIM29
Gene id 23650
Organism Homo sapiens
Official full symbol tripartite motif containing 29
Gene type protein-coding
Also known as ATDC
Summary The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.
Genomic regions Chromosome 11
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TRIM29-201 ENST00000341846.10 2977 588aa Protein coding CCDS8428 Q14134-1 NM_012101.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TRIM29-213 ENST00000529044.5 1233 327aa Protein coding CCDS81637 E9PRL4 - TSL:2, GENCODE basic,
TRIM29-210 ENST00000528870.5 2217 121aa Protein coding - E9PIQ2 - TSL:2, GENCODE basic,
TRIM29-219 ENST00000532833.1 555 89aa Protein coding - E9PI31 - CDS 3' incomplete, TSL:4,
TRIM29-214 ENST00000529495.1 551 95aa Protein coding - E9PLI4 - CDS 3' incomplete, TSL:5,
TRIM29-212 ENST00000529040.1 549 38aa Protein coding - E9PM74 - CDS 3' incomplete, TSL:4,
TRIM29-211 ENST00000529011.1 547 97aa Protein coding - E9PJ94 - CDS 3' incomplete, TSL:4,
TRIM29-220 ENST00000533302.5 514 169aa Protein coding - H0YF27 - CDS 5' incomplete, TSL:4,
TRIM29-221 ENST00000627238.1 510 169aa Protein coding - E9PJD4 - TSL:5, GENCODE basic,
TRIM29-209 ENST00000526881.1 385 94aa Protein coding - E9PPX1 - CDS 3' incomplete, TSL:3,
TRIM29-202 ENST00000475051.4 1702 169aa Nonsense mediated decay - E9PJD4 - TSL:5,
TRIM29-217 ENST00000531555.5 535 119aa Nonsense mediated decay - H0YD78 - CDS 5' incomplete, TSL:3,
TRIM29-204 ENST00000524816.7 1632 No protein Processed transcript - - - TSL:5,
TRIM29-207 ENST00000525887.5 965 No protein Processed transcript - - - TSL:2,
TRIM29-206 ENST00000525327.5 548 No protein Processed transcript - - - TSL:3,
TRIM29-205 ENST00000524956.4 314 No protein Processed transcript - - - TSL:5,
TRIM29-203 ENST00000524664.5 285 No protein Processed transcript - - - TSL:2,
TRIM29-208 ENST00000526161.5 1836 No protein Retained intron - - - TSL:1,
TRIM29-216 ENST00000530919.5 1227 No protein Retained intron - - - TSL:3,
TRIM29-215 ENST00000530470.1 771 No protein Retained intron - - - TSL:2,
TRIM29-218 ENST00000532195.1 673 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TRIM29
This KO Strategy loading
Red Cotton™ Notes Gene TRIM29 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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