CRISPR-U™ Gene Knockout Cell Line
Strategy
PLD3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PLD3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PLD3 |
| Gene id | 23646 |
| Organism | Homo sapiens |
| Official full symbol | phospholipase D family member 3 |
| Gene type | protein-coding |
| Also known as | AD19, HU-K4, HUK4, SCA46 |
| Summary | This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 14 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PLD3-206 | ENST00000409587.5 | 2180 | 490aa | Protein coding | CCDS33027 | A0A024R0Q4 Q8IV08 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PLD3-207 | ENST00000409735.9 | 2137 | 490aa | Protein coding | CCDS33027 | A0A024R0Q4 Q8IV08 | NM_012268.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PLD3-204 | ENST00000409281.5 | 2115 | 490aa | Protein coding | CCDS33027 | A0A024R0Q4 Q8IV08 | - | TSL:2, GENCODE basic, APPRIS P1, |
| PLD3-205 | ENST00000409419.5 | 1966 | 490aa | Protein coding | CCDS33027 | A0A024R0Q4 Q8IV08 | - | TSL:5, GENCODE basic, APPRIS P1, |
| PLD3-201 | ENST00000356508.9 | 1906 | 490aa | Protein coding | CCDS33027 | A0A024R0Q4 Q8IV08 | - | TSL:1, GENCODE basic, APPRIS P1, |
| PLD3-203 | ENST00000392032.6 | 873 | 128aa | Protein coding | - | A0A0A0MS28 | - | CDS 3' incomplete, TSL:5, |
| PLD3-217 | ENST00000594908.5 | 804 | 213aa | Protein coding | - | M0R1F7 | - | CDS 3' incomplete, TSL:3, |
| PLD3-202 | ENST00000359274.5 | 745 | 177aa | Protein coding | - | E2QRG1 | - | CDS 3' incomplete, TSL:3, |
| PLD3-219 | ENST00000598962.5 | 580 | 28aa | Protein coding | - | M0QZK2 | - | CDS 3' incomplete, TSL:4, |
| PLD3-221 | ENST00000599685.5 | 544 | 70aa | Protein coding | - | M0QY94 | - | CDS 3' incomplete, TSL:2, |
| PLD3-223 | ENST00000600948.5 | 536 | 44aa | Protein coding | - | M0R2E7 | - | CDS 3' incomplete, TSL:2, |
| PLD3-224 | ENST00000602131.5 | 503 | 119aa | Protein coding | - | M0QX99 | - | CDS 3' incomplete, TSL:2, |
| PLD3-218 | ENST00000596682.5 | 443 | 81aa | Protein coding | - | M0QX50 | - | CDS 3' incomplete, TSL:3, |
| PLD3-220 | ENST00000599353.1 | 332 | 111aa | Protein coding | - | M0R2W7 | - | CDS 5' and 3' incomplete, TSL:3, |
| PLD3-214 | ENST00000492243.1 | 710 | 121aa | Nonsense mediated decay | - | M0QYJ4 | - | CDS 5' incomplete, TSL:2, |
| PLD3-212 | ENST00000486134.1 | 327 | 71aa | Nonsense mediated decay | - | M0R3G9 | - | CDS 5' incomplete, TSL:2, |
| PLD3-216 | ENST00000594085.5 | 503 | No protein | Processed transcript | - | - | - | TSL:2, |
| PLD3-222 | ENST00000600479.5 | 477 | No protein | Processed transcript | - | - | - | TSL:4, |
| PLD3-211 | ENST00000485448.1 | 1308 | No protein | Retained intron | - | - | - | TSL:1, |
| PLD3-210 | ENST00000480030.1 | 903 | No protein | Retained intron | - | - | - | TSL:2, |
| PLD3-213 | ENST00000488311.1 | 750 | No protein | Retained intron | - | - | - | TSL:2, |
| PLD3-209 | ENST00000475983.1 | 731 | No protein | Retained intron | - | - | - | TSL:2, |
| PLD3-215 | ENST00000493006.1 | 694 | No protein | Retained intron | - | - | - | TSL:2, |
| PLD3-208 | ENST00000464586.1 | 253 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PLD3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PLD3 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: