CRISPR-U™ Gene Knockout Cell Line Strategy

PLD3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PLD3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PLD3
Gene id 23646
Organism Homo sapiens
Official full symbol phospholipase D family member 3
Gene type protein-coding
Also known as AD19, HU-K4, HUK4, SCA46
Summary This gene encodes a member of the phospholipase D (PLD) family of enzymes that catalyze the hydrolysis of membrane phospholipids. The encoded protein is a single-pass type II membrane protein and contains two PLD phosphodiesterase domains. This protein influences processing of amyloid-beta precursor protein. Mutations in this gene are associated with Alzheimer disease risk. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 19
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PLD3-206 ENST00000409587.5 2180 490aa Protein coding CCDS33027 A0A024R0Q4 Q8IV08 - TSL:5, GENCODE basic, APPRIS P1,
PLD3-207 ENST00000409735.9 2137 490aa Protein coding CCDS33027 A0A024R0Q4 Q8IV08 NM_012268.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PLD3-204 ENST00000409281.5 2115 490aa Protein coding CCDS33027 A0A024R0Q4 Q8IV08 - TSL:2, GENCODE basic, APPRIS P1,
PLD3-205 ENST00000409419.5 1966 490aa Protein coding CCDS33027 A0A024R0Q4 Q8IV08 - TSL:5, GENCODE basic, APPRIS P1,
PLD3-201 ENST00000356508.9 1906 490aa Protein coding CCDS33027 A0A024R0Q4 Q8IV08 - TSL:1, GENCODE basic, APPRIS P1,
PLD3-203 ENST00000392032.6 873 128aa Protein coding - A0A0A0MS28 - CDS 3' incomplete, TSL:5,
PLD3-217 ENST00000594908.5 804 213aa Protein coding - M0R1F7 - CDS 3' incomplete, TSL:3,
PLD3-202 ENST00000359274.5 745 177aa Protein coding - E2QRG1 - CDS 3' incomplete, TSL:3,
PLD3-219 ENST00000598962.5 580 28aa Protein coding - M0QZK2 - CDS 3' incomplete, TSL:4,
PLD3-221 ENST00000599685.5 544 70aa Protein coding - M0QY94 - CDS 3' incomplete, TSL:2,
PLD3-223 ENST00000600948.5 536 44aa Protein coding - M0R2E7 - CDS 3' incomplete, TSL:2,
PLD3-224 ENST00000602131.5 503 119aa Protein coding - M0QX99 - CDS 3' incomplete, TSL:2,
PLD3-218 ENST00000596682.5 443 81aa Protein coding - M0QX50 - CDS 3' incomplete, TSL:3,
PLD3-220 ENST00000599353.1 332 111aa Protein coding - M0R2W7 - CDS 5' and 3' incomplete, TSL:3,
PLD3-214 ENST00000492243.1 710 121aa Nonsense mediated decay - M0QYJ4 - CDS 5' incomplete, TSL:2,
PLD3-212 ENST00000486134.1 327 71aa Nonsense mediated decay - M0R3G9 - CDS 5' incomplete, TSL:2,
PLD3-216 ENST00000594085.5 503 No protein Processed transcript - - - TSL:2,
PLD3-222 ENST00000600479.5 477 No protein Processed transcript - - - TSL:4,
PLD3-211 ENST00000485448.1 1308 No protein Retained intron - - - TSL:1,
PLD3-210 ENST00000480030.1 903 No protein Retained intron - - - TSL:2,
PLD3-213 ENST00000488311.1 750 No protein Retained intron - - - TSL:2,
PLD3-209 ENST00000475983.1 731 No protein Retained intron - - - TSL:2,
PLD3-215 ENST00000493006.1 694 No protein Retained intron - - - TSL:2,
PLD3-208 ENST00000464586.1 253 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PLD3
This KO Strategy loading
Red Cotton™ Notes Gene PLD3 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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