CRISPR-U™ Gene Knockout Cell Line
Strategy
CORO1C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CORO1C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CORO1C |
| Gene id | 23603 |
| Organism | Homo sapiens |
| Official full symbol | coronin 1C |
| Gene type | protein-coding |
| Also known as | HCRNN4 |
| Summary | This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Three transcript variants encoding two different isoforms have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CORO1C-202 | ENST00000420959.6 | 3921 | 527aa | Protein coding | CCDS61236 | Q9ULV4-3 | - | TSL:1, GENCODE basic, |
| CORO1C-201 | ENST00000261401.8 | 3814 | 474aa | Protein coding | CCDS9120 | Q9ULV4-1 | NM_014325.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CORO1C-204 | ENST00000541050.5 | 2487 | 474aa | Protein coding | CCDS9120 | Q9ULV4-1 | - | TSL:2, GENCODE basic, APPRIS P1, |
| CORO1C-212 | ENST00000549772.5 | 1710 | 480aa | Protein coding | - | Q9ULV4-2 | - | TSL:1, GENCODE basic, |
| CORO1C-203 | ENST00000421578.6 | 1296 | 369aa | Protein coding | - | B4E3S0 | - | TSL:2, GENCODE basic, |
| CORO1C-205 | ENST00000546571.5 | 899 | 162aa | Protein coding | - | F8VSA4 | - | CDS 3' incomplete, TSL:4, |
| CORO1C-219 | ENST00000552871.5 | 587 | 125aa | Protein coding | - | F8VV53 | - | CDS 3' incomplete, TSL:3, |
| CORO1C-206 | ENST00000546705.5 | 577 | 165aa | Protein coding | - | H0YHL7 | - | CDS 5' incomplete, TSL:3, |
| CORO1C-215 | ENST00000551044.1 | 567 | 151aa | Protein coding | - | F8W1H8 | - | CDS 3' incomplete, TSL:3, |
| CORO1C-208 | ENST00000547294.5 | 566 | 181aa | Protein coding | - | F8VVB7 | - | CDS 3' incomplete, TSL:5, |
| CORO1C-213 | ENST00000550032.5 | 547 | 169aa | Protein coding | - | F8VTT6 | - | CDS 3' incomplete, TSL:2, |
| CORO1C-217 | ENST00000551550.5 | 547 | 163aa | Protein coding | - | F8VRE9 | - | CDS 3' incomplete, TSL:4, |
| CORO1C-214 | ENST00000550542.5 | 1245 | 159aa | Nonsense mediated decay | - | F8VUX3 | - | TSL:5, |
| CORO1C-210 | ENST00000549384.5 | 891 | No protein | Processed transcript | - | - | - | TSL:5, |
| CORO1C-218 | ENST00000552030.5 | 547 | No protein | Processed transcript | - | - | - | TSL:5, |
| CORO1C-211 | ENST00000549387.1 | 873 | No protein | Retained intron | - | - | - | TSL:3, |
| CORO1C-207 | ENST00000547170.1 | 804 | No protein | Retained intron | - | - | - | TSL:4, |
| CORO1C-209 | ENST00000547361.1 | 683 | No protein | Retained intron | - | - | - | TSL:2, |
| CORO1C-216 | ENST00000551059.1 | 474 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CORO1C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CORO1C had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: