CRISPR-U™ Gene Knockout Cell Line Strategy

DDX58 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DDX58 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DDX58
Gene id 23586
Organism Homo sapiens
Official full symbol DExD/H-box helicase 58
Gene type protein-coding
Also known as RIG-I, RIG1, RIGI, RLR-1, SGMRT2
Summary DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Genomic regions Chromosome 9
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DDX58-202 ENST00000379883.3 4628 925aa Protein coding CCDS6526 O95786-1 NM_014314.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DDX58-204 ENST00000679665.1 4961 854aa Protein coding - B3KWW1 - GENCODE basic,
DDX58-201 ENST00000379868.6 4493 880aa Protein coding - A2A376 - TSL:5,
DDX58-206 ENST00000679859.1 2118 425aa Protein coding - - - GENCODE basic,
DDX58-205 ENST00000679771.1 4492 142aa Nonsense mediated decay - - - -
DDX58-207 ENST00000680733.1 3617 76aa Nonsense mediated decay - - - -
DDX58-210 ENST00000681448.1 4635 No protein Retained intron - - - -
DDX58-209 ENST00000681352.1 3120 No protein Retained intron - - - -
DDX58-208 ENST00000680883.1 1282 No protein Retained intron - - - -
DDX58-203 ENST00000679662.1 661 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DDX58
This KO Strategy loading
Red Cotton™ Notes Gene DDX58 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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