CRISPR-U™ Gene Knockout Cell Line
Strategy
DDX58 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DDX58 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DDX58 |
| Gene id | 23586 |
| Organism | Homo sapiens |
| Official full symbol | DExD/H-box helicase 58 |
| Gene type | protein-coding |
| Also known as | RIG-I, RIG1, RIGI, RLR-1, SGMRT2 |
| Summary | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DDX58-202 | ENST00000379883.3 | 4628 | 925aa | Protein coding | CCDS6526 | O95786-1 | NM_014314.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DDX58-204 | ENST00000679665.1 | 4961 | 854aa | Protein coding | - | B3KWW1 | - | GENCODE basic, |
| DDX58-201 | ENST00000379868.6 | 4493 | 880aa | Protein coding | - | A2A376 | - | TSL:5, |
| DDX58-206 | ENST00000679859.1 | 2118 | 425aa | Protein coding | - | - | - | GENCODE basic, |
| DDX58-205 | ENST00000679771.1 | 4492 | 142aa | Nonsense mediated decay | - | - | - | - |
| DDX58-207 | ENST00000680733.1 | 3617 | 76aa | Nonsense mediated decay | - | - | - | - |
| DDX58-210 | ENST00000681448.1 | 4635 | No protein | Retained intron | - | - | - | - |
| DDX58-209 | ENST00000681352.1 | 3120 | No protein | Retained intron | - | - | - | - |
| DDX58-208 | ENST00000680883.1 | 1282 | No protein | Retained intron | - | - | - | - |
| DDX58-203 | ENST00000679662.1 | 661 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DDX58 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DDX58 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: