CRISPR-U™ Gene Knockout Cell Line Strategy

CDC42EP4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDC42EP4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC42EP4
Gene id 23580
Organism Homo sapiens
Official full symbol CDC42 effector protein 4
Gene type protein-coding
Also known as BORG4, CEP4, KAIA1777
Summary The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control.
Genomic regions Chromosome 17
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC42EP4-201 ENST00000335793.4 3098 356aa Protein coding CCDS11695 Q9H3Q1-1 NM_012121.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDC42EP4-202 ENST00000439510.2 2879 286aa Protein coding - Q9H3Q1-2 - TSL:2, GENCODE basic,
CDC42EP4-205 ENST00000580315.1 1022 127aa Protein coding - J3KRZ9 - CDS 3' incomplete, TSL:2,
CDC42EP4-206 ENST00000581014.1 737 79aa Protein coding - J3QR93 - TSL:5, GENCODE basic,
CDC42EP4-204 ENST00000579611.1 580 73aa Protein coding - J3QQS6 - CDS 3' incomplete, TSL:4,
CDC42EP4-209 ENST00000630622.1 240 79aa Protein coding - J3QR93 - TSL:5, GENCODE basic,
CDC42EP4-208 ENST00000582303.1 660 No protein Processed transcript - - - TSL:5,
CDC42EP4-203 ENST00000578516.1 572 No protein Processed transcript - - - TSL:4,
CDC42EP4-207 ENST00000581045.1 539 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC42EP4
This KO Strategy loading
Red Cotton™ Notes Gene CDC42EP4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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