CRISPR-U™ Gene Knockout Cell Line
Strategy
CDC42EP4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC42EP4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDC42EP4 |
| Gene id | 23580 |
| Organism | Homo sapiens |
| Official full symbol | CDC42 effector protein 4 |
| Gene type | protein-coding |
| Also known as | BORG4, CEP4, KAIA1777 |
| Summary | The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDC42EP4-201 | ENST00000335793.4 | 3098 | 356aa | Protein coding | CCDS11695 | Q9H3Q1-1 | NM_012121.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDC42EP4-202 | ENST00000439510.2 | 2879 | 286aa | Protein coding | - | Q9H3Q1-2 | - | TSL:2, GENCODE basic, |
| CDC42EP4-205 | ENST00000580315.1 | 1022 | 127aa | Protein coding | - | J3KRZ9 | - | CDS 3' incomplete, TSL:2, |
| CDC42EP4-206 | ENST00000581014.1 | 737 | 79aa | Protein coding | - | J3QR93 | - | TSL:5, GENCODE basic, |
| CDC42EP4-204 | ENST00000579611.1 | 580 | 73aa | Protein coding | - | J3QQS6 | - | CDS 3' incomplete, TSL:4, |
| CDC42EP4-209 | ENST00000630622.1 | 240 | 79aa | Protein coding | - | J3QR93 | - | TSL:5, GENCODE basic, |
| CDC42EP4-208 | ENST00000582303.1 | 660 | No protein | Processed transcript | - | - | - | TSL:5, |
| CDC42EP4-203 | ENST00000578516.1 | 572 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDC42EP4-207 | ENST00000581045.1 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDC42EP4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDC42EP4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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