CRISPR-U™ Gene Knockout Cell Line
Strategy
ARL2BP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ARL2BP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ARL2BP |
| Gene id | 23568 |
| Organism | Homo sapiens |
| Official full symbol | ADP ribosylation factor like GTPase 2 binding protein |
| Gene type | protein-coding |
| Also known as | BART, BART1, RP66 |
| Summary | ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ARL2BP-201 | ENST00000219204.8 | 1969 | 163aa | Protein coding | CCDS10776 | Q9Y2Y0-1 | NM_012106.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ARL2BP-203 | ENST00000563234.1 | 1416 | 171aa | Protein coding | - | H3BM52 | - | CDS 5' incomplete, TSL:2, |
| ARL2BP-202 | ENST00000562023.5 | 547 | 123aa | Protein coding | - | H3BU49 | - | TSL:3, GENCODE basic, |
| ARL2BP-204 | ENST00000565794.1 | 234 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ARL2BP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ARL2BP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: