CRISPR-U™ Gene Knockout Cell Line Strategy

ARL2BP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ARL2BP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ARL2BP
Gene id 23568
Organism Homo sapiens
Official full symbol ADP ribosylation factor like GTPase 2 binding protein
Gene type protein-coding
Also known as BART, BART1, RP66
Summary ADP-ribosylation factor (ARF)-like proteins (ARLs) comprise a functionally distinct group of the ARF family of RAS-related GTPases. The protein encoded by this gene binds to ARL2.GTP with high affinity but does not interact with ARL2.GDP, activated ARF, or RHO proteins. The lack of detectable membrane association of this protein or ARL2 upon activation of ARL2 is suggestive of actions distinct from those of the ARFs. This protein is considered to be the first ARL2-specific effector identified, due to its interaction with ARL2.GTP but lack of ARL2 GTPase-activating protein activity.
Genomic regions Chromosome 16
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ARL2BP-201 ENST00000219204.8 1969 163aa Protein coding CCDS10776 Q9Y2Y0-1 NM_012106.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ARL2BP-203 ENST00000563234.1 1416 171aa Protein coding - H3BM52 - CDS 5' incomplete, TSL:2,
ARL2BP-202 ENST00000562023.5 547 123aa Protein coding - H3BU49 - TSL:3, GENCODE basic,
ARL2BP-204 ENST00000565794.1 234 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ARL2BP
This KO Strategy loading
Red Cotton™ Notes Gene ARL2BP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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