CRISPR-U™ Gene Knockout Cell Line Strategy

FOS Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FOS Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FOS
Gene id 2353
Organism Homo sapiens
Official full symbol Fos proto-oncogene, AP-1 transcription factor subunit
Gene type protein-coding
Also known as AP-1, C-FOS, p55
Summary The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Genomic regions Chromosome 14
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FOS-201 ENST00000303562.9 2104 380aa Protein coding CCDS9841 P01100-1 NM_005252.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FOS-208 ENST00000555686.1 1496 266aa Protein coding - P01100-2 - TSL:2, GENCODE basic,
FOS-202 ENST00000535987.5 1402 344aa Protein coding - P01100-3 - TSL:2, GENCODE basic,
FOS-206 ENST00000555347.1 1280 232aa Protein coding - G3V2V7 - TSL:2, GENCODE basic,
FOS-207 ENST00000555672.1 834 142aa Protein coding - G3V5N7 - CDS 3' incomplete, TSL:2,
FOS-204 ENST00000554617.1 796 170aa Protein coding - G3V289 - TSL:2, GENCODE basic,
FOS-205 ENST00000555242.1 629 176aa Protein coding - G3V5J9 - TSL:2, GENCODE basic,
FOS-203 ENST00000554212.5 567 137aa Protein coding - G3V5N9 - CDS 3' incomplete, TSL:4,
FOS-210 ENST00000557139.1 249 83aa Protein coding - H0YJM3 - CDS 5' and 3' incomplete, TSL:5,
FOS-209 ENST00000556324.2 596 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FOS
This KO Strategy loading
Red Cotton™ Notes Gene FOS had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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