CRISPR-U™ Gene Knockout Cell Line
Strategy
FOS Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FOS Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FOS |
| Gene id | 2353 |
| Organism | Homo sapiens |
| Official full symbol | Fos proto-oncogene, AP-1 transcription factor subunit |
| Gene type | protein-coding |
| Also known as | AP-1, C-FOS, p55 |
| Summary | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FOS-201 | ENST00000303562.9 | 2104 | 380aa | Protein coding | CCDS9841 | P01100-1 | NM_005252.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FOS-208 | ENST00000555686.1 | 1496 | 266aa | Protein coding | - | P01100-2 | - | TSL:2, GENCODE basic, |
| FOS-202 | ENST00000535987.5 | 1402 | 344aa | Protein coding | - | P01100-3 | - | TSL:2, GENCODE basic, |
| FOS-206 | ENST00000555347.1 | 1280 | 232aa | Protein coding | - | G3V2V7 | - | TSL:2, GENCODE basic, |
| FOS-207 | ENST00000555672.1 | 834 | 142aa | Protein coding | - | G3V5N7 | - | CDS 3' incomplete, TSL:2, |
| FOS-204 | ENST00000554617.1 | 796 | 170aa | Protein coding | - | G3V289 | - | TSL:2, GENCODE basic, |
| FOS-205 | ENST00000555242.1 | 629 | 176aa | Protein coding | - | G3V5J9 | - | TSL:2, GENCODE basic, |
| FOS-203 | ENST00000554212.5 | 567 | 137aa | Protein coding | - | G3V5N9 | - | CDS 3' incomplete, TSL:4, |
| FOS-210 | ENST00000557139.1 | 249 | 83aa | Protein coding | - | H0YJM3 | - | CDS 5' and 3' incomplete, TSL:5, |
| FOS-209 | ENST00000556324.2 | 596 | No protein | Processed transcript | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FOS |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FOS had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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