CRISPR-U™ Gene Knockout Cell Line Strategy

SUZ12 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human SUZ12 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol SUZ12
Gene id 23512
Organism Homo sapiens
Official full symbol SUZ12 polycomb repressive complex 2 subunit
Gene type protein-coding
Also known as CHET9, IMMAS, JJAZ1
Summary This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region.
Genomic regions Chromosome 17
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
SUZ12-201 ENST00000322652.10 4495 739aa Protein coding CCDS11270 Q15022 NM_015355.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
SUZ12-204 ENST00000580398.1 3977 716aa Protein coding CCDS82101 J3QQW9 - TSL:1, GENCODE basic, APPRIS ALT1,
SUZ12-202 ENST00000494429.2 1895 No protein Retained intron - - - TSL:NA,
SUZ12-203 ENST00000578106.1 1367 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene SUZ12
This KO Strategy loading
Red Cotton™ Notes Gene SUZ12 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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