CRISPR-U™ Gene Knockout Cell Line Strategy

PIP5K1C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PIP5K1C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PIP5K1C
Gene id 23396
Organism Homo sapiens
Official full symbol phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
Gene type protein-coding
Also known as LCCS3, PIP5K-GAMMA, PIP5K1-gamma, PIP5Kgamma
Summary This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.
Genomic regions Chromosome 19
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PIP5K1C-201 ENST00000335312.8 5069 668aa Protein coding CCDS32872 O60331-1 NM_012398.3 TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
PIP5K1C-205 ENST00000589578.5 2933 707aa Protein coding CCDS74257 O60331-3 - TSL:1, GENCODE basic, APPRIS P4,
PIP5K1C-203 ENST00000539785.5 2289 640aa Protein coding CCDS56074 O60331-4 - TSL:2, GENCODE basic, APPRIS ALT2,
PIP5K1C-210 ENST00000679885.1 5069 694aa Protein coding - - - GENCODE basic, APPRIS ALT2,
PIP5K1C-202 ENST00000537021.1 3326 700aa Protein coding - O60331-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PIP5K1C-209 ENST00000679828.1 5223 204aa Nonsense mediated decay - - - -
PIP5K1C-204 ENST00000587482.1 1311 No protein Processed transcript - - - TSL:5,
PIP5K1C-206 ENST00000592530.1 456 No protein Processed transcript - - - TSL:5,
PIP5K1C-208 ENST00000637724.1 435 No protein Processed transcript - - - TSL:3,
PIP5K1C-207 ENST00000636612.1 153 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PIP5K1C
This KO Strategy loading
Red Cotton™ Notes Gene PIP5K1C had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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