CRISPR-U™ Gene Knockout Cell Line
Strategy
PIP5K1C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PIP5K1C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PIP5K1C |
| Gene id | 23396 |
| Organism | Homo sapiens |
| Official full symbol | phosphatidylinositol-4-phosphate 5-kinase type 1 gamma |
| Gene type | protein-coding |
| Also known as | LCCS3, PIP5K-GAMMA, PIP5K1-gamma, PIP5Kgamma |
| Summary | This locus encodes a type I phosphatidylinositol 4-phosphate 5-kinase. The encoded protein catalyzes phosphorylation of phosphatidylinositol 4-phosphate, producing phosphatidylinositol 4,5-bisphosphate. This enzyme is found at synapses and has been found to play roles in endocytosis and cell migration. Mutations at this locus have been associated with lethal congenital contractural syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PIP5K1C-201 | ENST00000335312.8 | 5069 | 668aa | Protein coding | CCDS32872 | O60331-1 | NM_012398.3 | TSL:1, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| PIP5K1C-205 | ENST00000589578.5 | 2933 | 707aa | Protein coding | CCDS74257 | O60331-3 | - | TSL:1, GENCODE basic, APPRIS P4, |
| PIP5K1C-203 | ENST00000539785.5 | 2289 | 640aa | Protein coding | CCDS56074 | O60331-4 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| PIP5K1C-210 | ENST00000679885.1 | 5069 | 694aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| PIP5K1C-202 | ENST00000537021.1 | 3326 | 700aa | Protein coding | - | O60331-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| PIP5K1C-209 | ENST00000679828.1 | 5223 | 204aa | Nonsense mediated decay | - | - | - | - |
| PIP5K1C-204 | ENST00000587482.1 | 1311 | No protein | Processed transcript | - | - | - | TSL:5, |
| PIP5K1C-206 | ENST00000592530.1 | 456 | No protein | Processed transcript | - | - | - | TSL:5, |
| PIP5K1C-208 | ENST00000637724.1 | 435 | No protein | Processed transcript | - | - | - | TSL:3, |
| PIP5K1C-207 | ENST00000636612.1 | 153 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PIP5K1C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PIP5K1C had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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