CRISPR-U™ Gene Knockout Cell Line
Strategy
EXOC7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EXOC7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EXOC7 |
| Gene id | 23265 |
| Organism | Homo sapiens |
| Official full symbol | exocyst complex component 7 |
| Gene type | protein-coding |
| Also known as | 2-5-3p, BLOM4, EX070, EXO70, EXOC1, Exo70p, YJL085W |
| Summary | The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EXOC7-216 | ENST00000589210.6 | 4782 | 684aa | Protein coding | CCDS32738 | Q9UPT5-1 | NM_001013839.4 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| EXOC7-201 | ENST00000332065.9 | 4596 | 653aa | Protein coding | CCDS11741 | Q9UPT5-2 | - | TSL:2, GENCODE basic, APPRIS ALT1, |
| EXOC7-202 | ENST00000335146.11 | 3519 | 735aa | Protein coding | CCDS45782 | Q9UPT5-3 | - | TSL:1, GENCODE basic, |
| EXOC7-220 | ENST00000607838.5 | 3353 | 656aa | Protein coding | CCDS45784 | B4DJ07 | - | TSL:5, GENCODE basic, |
| EXOC7-213 | ENST00000467929.6 | 2260 | 656aa | Protein coding | CCDS45784 | B4DJ07 | - | TSL:2, GENCODE basic, |
| EXOC7-221 | ENST00000634349.1 | 2124 | 707aa | Protein coding | CCDS45783 | Q9UPT5-6 | - | TSL:1, GENCODE basic, |
| EXOC7-207 | ENST00000411744.6 | 2031 | 676aa | Protein coding | CCDS45781 | Q9UPT5-5 | - | TSL:1, GENCODE basic, APPRIS P3, |
| EXOC7-206 | ENST00000406660.4 | 718 | 159aa | Protein coding | CCDS74164 | B5MCY9 | - | TSL:2, GENCODE basic, |
| EXOC7-205 | ENST00000405575.8 | 2208 | 693aa | Protein coding | - | A0A0A0MSB8 | - | TSL:5, GENCODE basic, |
| EXOC7-203 | ENST00000357231.8 | 1924 | 617aa | Protein coding | - | A0A0A0MRE1 | - | CDS 5' incomplete, TSL:1, |
| EXOC7-217 | ENST00000589507.6 | 820 | 168aa | Protein coding | - | K7ERQ5 | - | CDS 3' incomplete, TSL:5, |
| EXOC7-209 | ENST00000442951.5 | 583 | 121aa | Protein coding | - | C9JME6 | - | CDS 3' incomplete, TSL:2, |
| EXOC7-208 | ENST00000420116.1 | 543 | 122aa | Protein coding | - | C9JKC2 | - | CDS 3' incomplete, TSL:4, |
| EXOC7-219 | ENST00000592559.1 | 645 | 33aa | Nonsense mediated decay | - | K7ENP8 | - | CDS 5' incomplete, TSL:5, |
| EXOC7-218 | ENST00000591724.1 | 353 | No protein | Processed transcript | - | - | - | TSL:5, |
| EXOC7-211 | ENST00000465252.5 | 3336 | No protein | Retained intron | - | - | - | TSL:2, |
| EXOC7-204 | ENST00000405068.3 | 2958 | No protein | Retained intron | - | - | - | TSL:2, |
| EXOC7-212 | ENST00000467586.5 | 2393 | No protein | Retained intron | - | - | - | TSL:2, |
| EXOC7-210 | ENST00000460476.5 | 555 | No protein | Retained intron | - | - | - | TSL:4, |
| EXOC7-215 | ENST00000494787.6 | 542 | No protein | Retained intron | - | - | - | TSL:2, |
| EXOC7-214 | ENST00000486053.1 | 500 | No protein | Retained intron | - | - | - | TSL:3, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EXOC7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EXOC7 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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