CRISPR-U™ Gene Knockout Cell Line Strategy

EXOC7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EXOC7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EXOC7
Gene id 23265
Organism Homo sapiens
Official full symbol exocyst complex component 7
Gene type protein-coding
Also known as 2-5-3p, BLOM4, EX070, EXO70, EXOC1, Exo70p, YJL085W
Summary The protein encoded by this gene is a component of the exocyst complex. The exocyst complex plays a critical role in vesicular trafficking and the secretory pathway by targeting post-Golgi vesicles to the plasma membrane. The encoded protein is required for assembly of the exocyst complex and docking of the complex to the plasma membrane. The encoded protein may also play a role in pre-mRNA splicing through interactions with pre-mRNA-processing factor 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 4.
Genomic regions Chromosome 17
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EXOC7-216 ENST00000589210.6 4782 684aa Protein coding CCDS32738 Q9UPT5-1 NM_001013839.4 TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92,
EXOC7-201 ENST00000332065.9 4596 653aa Protein coding CCDS11741 Q9UPT5-2 - TSL:2, GENCODE basic, APPRIS ALT1,
EXOC7-202 ENST00000335146.11 3519 735aa Protein coding CCDS45782 Q9UPT5-3 - TSL:1, GENCODE basic,
EXOC7-220 ENST00000607838.5 3353 656aa Protein coding CCDS45784 B4DJ07 - TSL:5, GENCODE basic,
EXOC7-213 ENST00000467929.6 2260 656aa Protein coding CCDS45784 B4DJ07 - TSL:2, GENCODE basic,
EXOC7-221 ENST00000634349.1 2124 707aa Protein coding CCDS45783 Q9UPT5-6 - TSL:1, GENCODE basic,
EXOC7-207 ENST00000411744.6 2031 676aa Protein coding CCDS45781 Q9UPT5-5 - TSL:1, GENCODE basic, APPRIS P3,
EXOC7-206 ENST00000406660.4 718 159aa Protein coding CCDS74164 B5MCY9 - TSL:2, GENCODE basic,
EXOC7-205 ENST00000405575.8 2208 693aa Protein coding - A0A0A0MSB8 - TSL:5, GENCODE basic,
EXOC7-203 ENST00000357231.8 1924 617aa Protein coding - A0A0A0MRE1 - CDS 5' incomplete, TSL:1,
EXOC7-217 ENST00000589507.6 820 168aa Protein coding - K7ERQ5 - CDS 3' incomplete, TSL:5,
EXOC7-209 ENST00000442951.5 583 121aa Protein coding - C9JME6 - CDS 3' incomplete, TSL:2,
EXOC7-208 ENST00000420116.1 543 122aa Protein coding - C9JKC2 - CDS 3' incomplete, TSL:4,
EXOC7-219 ENST00000592559.1 645 33aa Nonsense mediated decay - K7ENP8 - CDS 5' incomplete, TSL:5,
EXOC7-218 ENST00000591724.1 353 No protein Processed transcript - - - TSL:5,
EXOC7-211 ENST00000465252.5 3336 No protein Retained intron - - - TSL:2,
EXOC7-204 ENST00000405068.3 2958 No protein Retained intron - - - TSL:2,
EXOC7-212 ENST00000467586.5 2393 No protein Retained intron - - - TSL:2,
EXOC7-210 ENST00000460476.5 555 No protein Retained intron - - - TSL:4,
EXOC7-215 ENST00000494787.6 542 No protein Retained intron - - - TSL:2,
EXOC7-214 ENST00000486053.1 500 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EXOC7
This KO Strategy loading
Red Cotton™ Notes Gene EXOC7 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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