CRISPR-U™ Gene Knockout Cell Line Strategy

DENND5A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DENND5A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DENND5A
Gene id 23258
Organism Homo sapiens
Official full symbol DENN domain containing 5A
Gene type protein-coding
Also known as EIEE49, RAB6IP1
Summary This gene encodes a DENN-domain-containing protein that functions as a RAB-activating guanine nucleotide exchange factor (GEF). This protein catalyzes the conversion of GDP to GTP and thereby converts inactive GDP-bound Rab proteins into their active GTP-bound form. The encoded protein is recruited by RAB6 onto Golgi membranes and is therefore referred to as RAB6-interacting protein 1. This protein binds with RAB39 as well. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene are associated with early infantile epileptic encephalopathy-49.
Genomic regions Chromosome 11
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DENND5A-201 ENST00000328194.8 4991 1287aa Protein coding CCDS31423 Q6IQ26-1 NM_015213.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
DENND5A-209 ENST00000530044.5 4060 1241aa Protein coding CCDS58119 Q6IQ26-2 - TSL:2, GENCODE basic,
DENND5A-219 ENST00000679568.1 4796 1307aa Protein coding - - - GENCODE basic,
DENND5A-236 ENST00000681203.1 4710 1263aa Protein coding - - - GENCODE basic, APPRIS ALT2,
DENND5A-225 ENST00000680294.1 4527 1218aa Protein coding - - - GENCODE basic,
DENND5A-215 ENST00000533737.5 743 195aa Protein coding - H0YEA8 - CDS 5' incomplete, TSL:3,
DENND5A-207 ENST00000528725.5 728 186aa Protein coding - H0YDS8 - CDS 5' incomplete, TSL:3,
DENND5A-202 ENST00000524446.2 566 189aa Protein coding - H0YCW5 - CDS 5' and 3' incomplete, TSL:4,
DENND5A-223 ENST00000679999.1 5182 1136aa Nonsense mediated decay - - - -
DENND5A-210 ENST00000530780.2 4765 69aa Nonsense mediated decay - E9PIS3 - TSL:3,
DENND5A-228 ENST00000680554.1 4643 1031aa Nonsense mediated decay - - - -
DENND5A-231 ENST00000680742.1 4611 1127aa Nonsense mediated decay - - - -
DENND5A-227 ENST00000680470.1 4509 660aa Nonsense mediated decay - - - -
DENND5A-224 ENST00000680252.1 4358 873aa Nonsense mediated decay - - - CDS 5' incomplete,
DENND5A-234 ENST00000681158.1 4272 873aa Nonsense mediated decay - - - CDS 5' incomplete,
DENND5A-204 ENST00000526707.5 2861 807aa Nonsense mediated decay - B4DFB6 - TSL:2,
DENND5A-206 ENST00000527896.1 604 104aa Nonsense mediated decay - H0YDV6 - CDS 5' incomplete, TSL:3,
DENND5A-230 ENST00000680599.1 4736 No protein Processed transcript - - - -
DENND5A-226 ENST00000680358.1 3996 No protein Processed transcript - - - -
DENND5A-235 ENST00000681173.1 3447 No protein Processed transcript - - - -
DENND5A-229 ENST00000680576.1 8203 No protein Retained intron - - - -
DENND5A-216 ENST00000679446.1 6692 No protein Retained intron - - - -
DENND5A-233 ENST00000680885.1 6376 No protein Retained intron - - - -
DENND5A-217 ENST00000679458.1 6097 No protein Retained intron - - - -
DENND5A-222 ENST00000679926.1 5996 No protein Retained intron - - - -
DENND5A-218 ENST00000679460.1 5750 No protein Retained intron - - - -
DENND5A-220 ENST00000679745.1 5199 No protein Retained intron - - - -
DENND5A-238 ENST00000681425.1 5166 No protein Retained intron - - - -
DENND5A-237 ENST00000681371.1 4560 No protein Retained intron - - - -
DENND5A-213 ENST00000531747.2 4367 No protein Retained intron - - - TSL:3,
DENND5A-211 ENST00000530867.2 4278 No protein Retained intron - - - TSL:2,
DENND5A-221 ENST00000679773.1 3855 No protein Retained intron - - - -
DENND5A-205 ENST00000527700.5 3593 No protein Retained intron - - - TSL:2,
DENND5A-232 ENST00000680791.1 3572 No protein Retained intron - - - -
DENND5A-239 ENST00000681639.1 2965 No protein Retained intron - - - -
DENND5A-208 ENST00000529977.5 2606 No protein Retained intron - - - TSL:2,
DENND5A-203 ENST00000525784.6 2553 No protein Retained intron - - - TSL:3,
DENND5A-240 ENST00000681915.1 2241 No protein Retained intron - - - -
DENND5A-214 ENST00000532696.2 2220 No protein Retained intron - - - TSL:2,
DENND5A-212 ENST00000531580.1 564 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DENND5A
This KO Strategy loading
Red Cotton™ Notes Gene DENND5A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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