CRISPR-U™ Gene Knockout Cell Line
Strategy
NUP210 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NUP210 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NUP210 |
| Gene id | 23225 |
| Organism | Homo sapiens |
| Official full symbol | nucleoporin 210 |
| Gene type | protein-coding |
| Also known as | GP210, POM210 |
| Summary | The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NUP210-201 | ENST00000254508.7 | 7206 | 1887aa | Protein coding | CCDS33704 | Q8TEM1-1 | NM_024923.4 | TSL:2, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NUP210-204 | ENST00000485755.1 | 474 | No protein | Processed transcript | - | - | - | TSL:5, |
| NUP210-202 | ENST00000420141.2 | 3134 | No protein | Retained intron | - | - | - | TSL:1, |
| NUP210-203 | ENST00000479519.5 | 1312 | No protein | Retained intron | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NUP210 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NUP210 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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