CRISPR-U™ Gene Knockout Cell Line Strategy

FLT3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FLT3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FLT3
Gene id 2322
Organism Homo sapiens
Official full symbol fms related receptor tyrosine kinase 3
Gene type protein-coding
Also known as CD135, FLK-2, FLK2, STK1
Summary This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Genomic regions Chromosome 13
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FLT3-201 ENST00000241453.12 3826 993aa Protein coding CCDS31953 P36888-1 NM_004119.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FLT3-202 ENST00000380987.2 3634 739aa Nonsense mediated decay - E7ER61 - TSL:1,
FLT3-203 ENST00000469894.1 474 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FLT3
This KO Strategy loading
Red Cotton™ Notes Gene FLT3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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