CRISPR-U™ Gene Knockout Cell Line
Strategy
JMJD6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human JMJD6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | JMJD6 |
| Gene id | 23210 |
| Organism | Homo sapiens |
| Official full symbol | jumonji domain containing 6, arginine demethylase and lysine hydroxylase |
| Gene type | protein-coding |
| Also known as | PSR, PTDSR, PTDSR1 |
| Summary | This gene encodes a nuclear protein with a JmjC domain. JmjC domain-containing proteins are predicted to function as protein hydroxylases or histone demethylases. This protein was first identified as a putative phosphatidylserine receptor involved in phagocytosis of apoptotic cells; however, subsequent studies have indicated that it does not directly function in the clearance of apoptotic cells, and questioned whether it is a true phosphatidylserine receptor. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| JMJD6-203 | ENST00000445478.6 | 5303 | 414aa | Protein coding | CCDS42383 | Q6NYC1-3 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| JMJD6-202 | ENST00000397625.9 | 1621 | 403aa | Protein coding | CCDS42384 | Q6NYC1-1 | NM_015167.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| JMJD6-208 | ENST00000617192.4 | 5517 | 361aa | Protein coding | - | B2WTI4 | - | TSL:5, GENCODE basic, |
| JMJD6-205 | ENST00000585429.1 | 1330 | 395aa | Protein coding | - | K7EJU9 | - | TSL:5, GENCODE basic, |
| JMJD6-204 | ENST00000542934.5 | 1898 | 361aa | Nonsense mediated decay | - | B2WTI4 | - | TSL:2, |
| JMJD6-201 | ENST00000303996.10 | 1893 | 335aa | Nonsense mediated decay | - | B2WTI3 | - | TSL:2, |
| JMJD6-207 | ENST00000591460.1 | 667 | No protein | Retained intron | - | - | - | TSL:2, |
| JMJD6-206 | ENST00000589982.1 | 467 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | JMJD6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene JMJD6 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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