CRISPR-U™ Gene Knockout Cell Line Strategy

PSME4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PSME4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PSME4
Gene id 23198
Organism Homo sapiens
Official full symbol proteasome activator subunit 4
Gene type protein-coding
Also known as PA200
Genomic regions Chromosome 2
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PSME4-202 ENST00000404125.6 7250 1843aa Protein coding CCDS33197 Q14997-1 NM_014614.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PSME4-201 ENST00000389993.7 6581 571aa Nonsense mediated decay - F8WBH5 - TSL:1,
PSME4-206 ENST00000476586.5 1422 No protein Processed transcript - - - TSL:1,
PSME4-208 ENST00000481518.1 886 No protein Processed transcript - - - TSL:5,
PSME4-204 ENST00000466539.1 548 No protein Processed transcript - - - TSL:4,
PSME4-209 ENST00000488687.5 3073 No protein Retained intron - - - TSL:2,
PSME4-203 ENST00000461810.1 562 No protein Retained intron - - - TSL:3,
PSME4-205 ENST00000475694.1 547 No protein Retained intron - - - TSL:3,
PSME4-207 ENST00000478731.1 514 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PSME4
This KO Strategy loading
Red Cotton™ Notes Gene PSME4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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