CRISPR-U™ Gene Knockout Cell Line
Strategy
PSME4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PSME4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PSME4 |
| Gene id | 23198 |
| Organism | Homo sapiens |
| Official full symbol | proteasome activator subunit 4 |
| Gene type | protein-coding |
| Also known as | PA200 |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PSME4-202 | ENST00000404125.6 | 7250 | 1843aa | Protein coding | CCDS33197 | Q14997-1 | NM_014614.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PSME4-201 | ENST00000389993.7 | 6581 | 571aa | Nonsense mediated decay | - | F8WBH5 | - | TSL:1, |
| PSME4-206 | ENST00000476586.5 | 1422 | No protein | Processed transcript | - | - | - | TSL:1, |
| PSME4-208 | ENST00000481518.1 | 886 | No protein | Processed transcript | - | - | - | TSL:5, |
| PSME4-204 | ENST00000466539.1 | 548 | No protein | Processed transcript | - | - | - | TSL:4, |
| PSME4-209 | ENST00000488687.5 | 3073 | No protein | Retained intron | - | - | - | TSL:2, |
| PSME4-203 | ENST00000461810.1 | 562 | No protein | Retained intron | - | - | - | TSL:3, |
| PSME4-205 | ENST00000475694.1 | 547 | No protein | Retained intron | - | - | - | TSL:3, |
| PSME4-207 | ENST00000478731.1 | 514 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PSME4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PSME4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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