CRISPR-U™ Gene Knockout Cell Line Strategy

ATG4B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG4B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG4B
Gene id 23192
Organism Homo sapiens
Official full symbol autophagy related 4B cysteine peptidase
Gene type protein-coding
Also known as APG4B, AUTL1
Summary Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Genomic regions Chromosome 2
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG4B-206 ENST00000405546.7 3294 380aa Protein coding CCDS46565 Q9Y4P1-6 - TSL:2, GENCODE basic,
ATG4B-205 ENST00000404914.8 2797 393aa Protein coding CCDS46564 Q9Y4P1-1 NM_013325.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATG4B-204 ENST00000402096.5 1685 331aa Protein coding - Q9Y4P1-4 - TSL:2, GENCODE basic,
ATG4B-202 ENST00000400771.7 663 219aa Protein coding - C9JIK8 - CDS 3' incomplete, TSL:5,
ATG4B-208 ENST00000419606.5 553 66aa Protein coding - C9J1C1 - CDS 3' incomplete, TSL:4,
ATG4B-226 ENST00000625810.2 370 88aa Protein coding - F2Z2K8 - TSL:4, GENCODE basic,
ATG4B-201 ENST00000344376.9 1566 76aa Nonsense mediated decay - H0Y2Y0 - CDS 5' incomplete, TSL:5,
ATG4B-203 ENST00000400772.6 625 88aa Nonsense mediated decay - F2Z2K8 - TSL:4,
ATG4B-207 ENST00000415107.5 356 88aa Nonsense mediated decay - F2Z2K8 - TSL:5,
ATG4B-212 ENST00000430617.6 623 No protein Processed transcript - - - TSL:3,
ATG4B-222 ENST00000491867.5 435 No protein Processed transcript - - - TSL:5,
ATG4B-220 ENST00000482507.5 2414 No protein Retained intron - - - TSL:1,
ATG4B-225 ENST00000494465.5 2365 No protein Retained intron - - - TSL:5,
ATG4B-217 ENST00000475693.1 2237 No protein Retained intron - - - TSL:2,
ATG4B-221 ENST00000483778.5 1103 No protein Retained intron - - - TSL:3,
ATG4B-223 ENST00000493618.5 899 No protein Retained intron - - - TSL:5,
ATG4B-213 ENST00000460211.5 871 No protein Retained intron - - - TSL:2,
ATG4B-224 ENST00000494132.1 828 No protein Retained intron - - - TSL:2,
ATG4B-209 ENST00000425239.5 807 No protein Retained intron - - - TSL:2,
ATG4B-216 ENST00000475195.5 762 No protein Retained intron - - - TSL:2,
ATG4B-215 ENST00000468018.5 721 No protein Retained intron - - - TSL:5,
ATG4B-214 ENST00000465399.5 581 No protein Retained intron - - - TSL:2,
ATG4B-210 ENST00000428861.1 580 No protein Retained intron - - - TSL:2,
ATG4B-218 ENST00000479554.5 556 No protein Retained intron - - - TSL:4,
ATG4B-211 ENST00000429899.1 524 No protein Retained intron - - - TSL:4,
ATG4B-219 ENST00000479941.1 374 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG4B
This KO Strategy loading
Red Cotton™ Notes Gene ATG4B had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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