CRISPR-U™ Gene Knockout Cell Line Strategy

MPRIP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPRIP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MPRIP
Gene id 23164
Organism Homo sapiens
Official full symbol myosin phosphatase Rho interacting protein
Gene type protein-coding
Also known as M-RIP, MRIP, RHOIP3, RIP3, p116Rip
Genomic regions Chromosome 17
Strategy Summary
This gene has 14 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MPRIP-205 ENST00000395811.9 10960 1038aa Protein coding CCDS42268 Q6WCQ1-2 - TSL:1, GENCODE basic, APPRIS ALT2,
MPRIP-202 ENST00000341712.8 3846 1025aa Protein coding CCDS32578 Q6WCQ1-1 - TSL:5, GENCODE basic, APPRIS ALT2,
MPRIP-221 ENST00000651222.2 15419 2425aa Protein coding - A0A494BZV2 NM_001364716.4 GENCODE basic, APPRIS P3, MANE Select v0.92,
MPRIP-201 ENST00000313485.10 6050 1794aa Protein coding - H0Y2S9 - CDS 5' incomplete, TSL:5,
MPRIP-206 ENST00000414263.5 4043 1091aa Protein coding - H0Y7E2 - CDS 5' incomplete, TSL:2,
MPRIP-219 ENST00000584067.5 3195 844aa Protein coding - J3KSW8 - CDS 5' incomplete, TSL:1,
MPRIP-207 ENST00000429184.7 914 277aa Protein coding - H7C3G6 - CDS 5' incomplete, TSL:5,
MPRIP-209 ENST00000466186.2 880 294aa Protein coding - A0A1W2PPU9 - CDS 5' and 3' incomplete, TSL:5,
MPRIP-214 ENST00000579361.1 844 59aa Protein coding - J3QL44 - CDS 5' incomplete, TSL:3,
MPRIP-211 ENST00000578209.5 751 181aa Protein coding - J3QRL2 - CDS 5' incomplete, TSL:3,
MPRIP-203 ENST00000395806.5 527 63aa Protein coding - A8MZF8 - TSL:3, GENCODE basic,
MPRIP-210 ENST00000577514.5 513 139aa Protein coding - J3KSK7 - CDS 3' incomplete, TSL:5,
MPRIP-215 ENST00000579832.1 438 28aa Protein coding - K7EJ14 - CDS 5' incomplete, TSL:3,
MPRIP-208 ENST00000462033.1 309 95aa Protein coding - K7EL39 - CDS 5' incomplete, TSL:2,
MPRIP-204 ENST00000395807.2 926 No protein Processed transcript - - - TSL:2,
MPRIP-213 ENST00000578548.1 756 No protein Processed transcript - - - TSL:4,
MPRIP-212 ENST00000578521.1 569 No protein Processed transcript - - - TSL:4,
MPRIP-220 ENST00000584936.2 490 No protein Processed transcript - - - TSL:5,
MPRIP-218 ENST00000581989.1 444 No protein Processed transcript - - - TSL:5,
MPRIP-216 ENST00000581855.1 329 No protein Processed transcript - - - TSL:2,
MPRIP-217 ENST00000581955.1 868 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MPRIP
This KO Strategy loading
Red Cotton™ Notes Gene MPRIP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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