CRISPR-U™ Gene Knockout Cell Line
Strategy
KDM6B Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KDM6B Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KDM6B |
| Gene id | 23135 |
| Organism | Homo sapiens |
| Official full symbol | lysine demethylase 6B |
| Gene type | protein-coding |
| Also known as | JMJD3, NEDCFSA |
| Summary | The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KDM6B-202 | ENST00000448097.7 | 6728 | 1643aa | Protein coding | CCDS86569 | O15054-2 | NM_001348716.2 | TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| KDM6B-201 | ENST00000254846.9 | 6713 | 1682aa | Protein coding | CCDS32552 | O15054-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| KDM6B-203 | ENST00000570632.1 | 1339 | 361aa | Protein coding | - | I3L0Z0 | - | CDS 3' incomplete, TSL:5, |
| KDM6B-205 | ENST00000572030.1 | 432 | No protein | Processed transcript | - | - | - | TSL:3, |
| KDM6B-204 | ENST00000571047.5 | 362 | No protein | Processed transcript | - | - | - | TSL:5, |
| KDM6B-206 | ENST00000575521.1 | 219 | No protein | Processed transcript | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KDM6B |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KDM6B had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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