CRISPR-U™ Gene Knockout Cell Line Strategy

KDM6B Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KDM6B Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KDM6B
Gene id 23135
Organism Homo sapiens
Official full symbol lysine demethylase 6B
Gene type protein-coding
Also known as JMJD3, NEDCFSA
Summary The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 17
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KDM6B-202 ENST00000448097.7 6728 1643aa Protein coding CCDS86569 O15054-2 NM_001348716.2 TSL:5, GENCODE basic, APPRIS ALT2, MANE Select v0.92,
KDM6B-201 ENST00000254846.9 6713 1682aa Protein coding CCDS32552 O15054-1 - TSL:1, GENCODE basic, APPRIS P4,
KDM6B-203 ENST00000570632.1 1339 361aa Protein coding - I3L0Z0 - CDS 3' incomplete, TSL:5,
KDM6B-205 ENST00000572030.1 432 No protein Processed transcript - - - TSL:3,
KDM6B-204 ENST00000571047.5 362 No protein Processed transcript - - - TSL:5,
KDM6B-206 ENST00000575521.1 219 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KDM6B
This KO Strategy loading
Red Cotton™ Notes Gene KDM6B had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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