CRISPR-U™ Gene Knockout Cell Line
Strategy
ATG2A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATG2A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATG2A |
| Gene id | 23130 |
| Organism | Homo sapiens |
| Official full symbol | autophagy related 2A |
| Gene type | protein-coding |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATG2A-201 | ENST00000377264.8 | 6318 | 1938aa | Protein coding | CCDS31602 | Q2TAZ0-1 | NM_015104.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATG2A-202 | ENST00000418259.5 | 5654 | 1742aa | Protein coding | - | H7C3T2 | - | CDS 5' incomplete, TSL:5, |
| ATG2A-203 | ENST00000421419.3 | 1481 | 331aa | Protein coding | - | Q2TAZ0-4 | - | TSL:1, GENCODE basic, |
| ATG2A-204 | ENST00000461701.5 | 2031 | No protein | Retained intron | - | - | - | TSL:2, |
| ATG2A-205 | ENST00000461955.1 | 575 | No protein | Retained intron | - | - | - | TSL:4, |
| ATG2A-206 | ENST00000472525.1 | 515 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATG2A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATG2A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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