CRISPR-U™ Gene Knockout Cell Line Strategy

CDK19 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDK19 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK19
Gene id 23097
Organism Homo sapiens
Official full symbol cyclin dependent kinase 19
Gene type protein-coding
Also known as CDC2L6, CDK11, EIEE87, bA346C16.3
Summary This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 6
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK19-202 ENST00000368911.8 6399 502aa Protein coding CCDS5085 Q9BWU1-1 NM_015076.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDK19-201 ENST00000323817.7 6067 442aa Protein coding CCDS75503 Q9BWU1-2 - TSL:1, GENCODE basic,
CDK19-203 ENST00000413605.6 6007 398aa Protein coding - F6QTA4 - TSL:1, GENCODE basic,
CDK19-204 ENST00000457688.5 955 251aa Protein coding - Q5JQZ9 - CDS 3' incomplete, TSL:5,
CDK19-206 ENST00000463016.1 604 122aa Nonsense mediated decay - H0YDW9 - CDS 5' incomplete, TSL:5,
CDK19-208 ENST00000497709.1 1117 No protein Processed transcript - - - TSL:3,
CDK19-207 ENST00000468997.5 1083 No protein Processed transcript - - - TSL:1,
CDK19-205 ENST00000460913.1 448 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK19
This KO Strategy loading
Red Cotton™ Notes Gene CDK19 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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