CRISPR-U™ Gene Knockout Cell Line Strategy

PEG10 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PEG10 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PEG10
Gene id 23089
Organism Homo sapiens
Official full symbol paternally expressed 10
Gene type protein-coding
Also known as EDR, HB-1, MEF3L, Mar2, Mart2, RGAG3, RTL2, SIRH1
Summary This is a paternally expressed imprinted gene that is thought to have been derived from the Ty3/Gypsy family of retrotransposons. It contains two overlapping open reading frames, RF1 and RF2, and expresses two proteins: a shorter, gag-like protein (with a CCHC-type zinc finger domain) from RF1; and a longer, gag/pol-like fusion protein (with an additional aspartic protease motif) from RF1/RF2 by -1 translational frameshifting (-1 FS). While -1 FS has been observed in RNA viruses and transposons in both prokaryotes and eukaryotes, this gene represents the first example of -1 FS in a eukaryotic cellular gene. This gene is highly conserved across mammalian species and retains the heptanucleotide (GGGAAAC) and pseudoknot elements required for -1 FS. It is expressed in adult and embryonic tissues (most notably in placenta) and reported to have a role in cell proliferation, differentiation and apoptosis. Overexpression of this gene has been associated with several malignancies, such as hepatocellular carcinoma and B-cell lymphocytic leukemia. Knockout mice lacking this gene showed early embryonic lethality with placental defects, indicating the importance of this gene in embryonic development. Additional isoforms resulting from alternatively spliced transcript variants, and use of upstream non-AUG (CUG) start codon have been reported for this gene.
Genomic regions Chromosome 7
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PEG10-202 ENST00000482108.1 6618 325aa Protein coding CCDS55126 Q86TG7-2 - TSL:1, GENCODE basic, APPRIS ALT2,
PEG10-208 ENST00000615790.5 6394 359aa Protein coding CCDS75637 A0A087WZG9 - TSL:1, GENCODE basic, APPRIS ALT2,
PEG10-203 ENST00000488574.5 2587 401aa Protein coding CCDS75636 B4DSP0 - TSL:2, GENCODE basic, APPRIS P4,
PEG10-206 ENST00000612941.2 6392 741aa Protein coding - A0A087WUL4 - TSL:5, GENCODE basic, APPRIS ALT2,
PEG10-209 ENST00000617526.4 6392 707aa Protein coding - A0A087WXK2 - TSL:5, GENCODE basic, APPRIS ALT2,
PEG10-205 ENST00000612748.1 2585 783aa Protein coding - A0A087WX23 - TSL:5, GENCODE basic, APPRIS ALT2,
PEG10-207 ENST00000613043.1 574 31aa Protein coding - A0A087WYS2 - CDS 3' incomplete, TSL:5,
PEG10-201 ENST00000465184.1 788 No protein Processed transcript - - - TSL:3,
PEG10-204 ENST00000493935.1 763 No protein Processed transcript - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PEG10
This KO Strategy loading
Red Cotton™ Notes Gene PEG10 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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